色谱知识教案分析.ppt

One of the most common problems (mistakes!) made in method development is the use of 缓冲液s at the incorrect pH range. Often methods are written where a 缓冲液 is used outside of its pH range! Be sure to use the proper 缓冲液 for the proper pH. At pH 2.5, partially ionized strong acids (0.1% phosphoric acid, TFA) can function as effective pH stabilizers. Formate, acetate, TFA and ammonia are volatile, making them a good choice for LC/MS. Volatile (but weak) 缓冲液s can be made by combining salts. Ammonium formate will 缓冲液 weakly between pH 3.8-4.8 and 8.2-10.2. Ammonium acetate will 缓冲液 weakly