示范论文邹坦坦-紫扇贝线粒体coxⅠ基因片段克隆及序列分析汇总.doc

青岛农业大学 毕业论文 题 目： 紫扇贝线粒体coxⅠ基因片段克隆及序列分析 姓 名： 邹坦坦 学 院： 海洋科学与工程学院 专 业： 水产养殖 班 级： 09.2 学 号： 指导教师： 李朝霞 目录 中文摘要····························································1 Abstract····························································1 引言································································2 1 材料与方法························································3 1.1 实验材料························································3 1.2 实验试剂························································3 1.3 方法····························································3 1.3.1 简并引物设计··················································3 1.3.2 DNA提取·······················································4 1.3.3 PCR扩增······················································4 1.3.4 染色及凝胶电泳················································5 1.3.5 染色拍照······················································5 1.3.6 DNA回收·······················································5 1.3.7 连接··························································5 1.3.8 转化··························································5 1.3.9 挑单菌落培养测序··············································6 1.3.10 测序及序列分析···············································6 2结果与分析························································6 2.1 基因组DNA提取··················································6 2.2 PCR扩增························································7 2.3 紫扇贝coxⅠ基因测序结果·········································7 2.4 同源性对比······················································8 2.5 构建系统进化树·················································11 3 讨论·····························································12 参考文献···················································