《生物信息教学课件》cancer_systemsbiology.pptVIP

More sensitive technology is needed for delineation of networks perturbed in diseases Digital gene expression mRNA AAAA AAAA AAAA Tagged cDNA signatures 3’-most DpnII site 106 Megaclone cDNA signatures - 100,000 copies per bead Sequence 20 nucleotides on 106 beads simultaneously Count signature sequences to establish gene expression profile Brenner, S., M. Nat Biotechnol 18 6 : 630-4. Many Applications - One Platform DNA Sequencing Whole genome re-sequencing Expression Analysis mRNA Small RNAs Other Applications DNaseI hypersensitive site mapping Chromatin Immunoprecipitation ChIP