Arabidopsis 1-Aminocyclopropane-1-Carboxylate Synthase Gene.doc

Arabidopsis 1-Aminocyclopropane-1-Carboxylate Synthase Gene Arabidopsis 1-Aminocyclopropane-1-Carboxylate Synthase Gene AtACS7 Is a System 2 ACS Gene WU Shuang 1, LIN Jingyu 1, CHANG Li 1, SHIH Ming-Che 2, WANG Ningning 1,* 1 Department of Plant Biology and Ecology, Nankai University, Tianjin 300071, P. R. China 2 114 Biology Building, Department of Biological Sciences and Roy J. Carver Center for Comparative Genomics, University of Iowa, Iowa City, Iowa 52242, USA *Corresponding Author: Tel: +86 (0)22 2350 3714; Fax: +86 (0)22 2350 8800. E-mail address: wangnn@nankai.edu.cn Footnote: This work was financially supported by a grant from the Natural Science Foundation of China (NSFC, No. and the Program for New Century Excellent Talents in University (grants No. NCET-05-0224) to Ning Ning Wang. 1  Abstract 1-Aminocyclopropane-1-carboxylate synthase (ACC synthase, ACS) (S-adenosyl-L-methionine methylthioadenosine-lyase; EC 4.4.1.14), the key enzyme for ethylene biosynthesis, is subjected to positive or negative feedback regulation by the hormone itself. Here we report, aided by promoter-GUS ( β -glucuronidase) reporter approach, that among the multigene family of Arabidopsis ACC synthases, AtACS5 and AtACS7 exhibited active expression in similar localizations but quite different responses to ethylene. The AtACS5::GUS and AtACS7::GUS fusions were found to express in company during the entire life cycle of transgenic Arabidopsis. However, treating the 2-week-old light-grown seedlings with exogenous ethylene greatly increased the GUS activity driven by AtACS7 promoter but exhibited no effect on the expression level of AtACS5::GUS transgene. The highest expression level of AtACS5::GUS fusion was detected in the 3-d-old etiolated seedlings, and the developmental expression profile of AtACS5 was not affected by the etr1-1 mutation. In contrast, the expression of AtACS7 during the entire life cycle of Arabidopsis, such as in 3-d-old etiol