Givingagoodpresentation浅析.ppt

EZH2 inhibitors treatments enhance NKG2D expression on human NK cells NKG2D expression is regulated by EZH2 mediated H3K27me3 modification on its gene loci and is essential for EZH2 inhibition improved NK cells development EZH2 inhibitors in vitro treatments enhance the NKG2D dependent killing ability of mouse NK cells against Yac-1 cells EZH2 inhibitors in vitro treatments enhance the NKG2D dependent killing ability of human NK cells against K562 cells EZH2 regulates NK cells development and function via repressing the expression of NKG2D Summary EZH2 regulates NK cell commitment, development and function in both human and mouse. NKG2D is one of EZH2 targets and key functional mediators in NK cells. We established very reliable and efficient in vivo and in vitro research platforms to investigate NK cell biology and mechanisms. Ongoing/Future work Systematic exploration of mechanisms underlying EZH2 function in NK cell. Identify other histone methylases and demethylases important in NK cells. Long-term goals Good timing; excellent research system and platform; right research strategy. NK Cell Biology -- Regulatory network in NK cell commitment, development, and function. -- Identify key factors: unique, functional, druggable. NK Cell clinic -- Immunotherapy against cancer. -- Treatment of virus infection. -- Prevention and treatment of autoimmune diseases. 研究目标 组蛋白 甲基化酶 Cis-Factor RNA-Seq ChIP-Seq Trans-Factor CO-IP Mass-Spec Crystal structure 关于NK细胞 的调控网络 HSC NK Fucntional NK Development Education Memory NK Activation Unique Functional Druggable Acknowledgement Collaborators Harvard Medical School Charlie Roberts Labortory Stu Orkin Labortory Harvey Cantor Labortory Shirley Xiaole Liu Labortory Christine Lian Labortory Jiping Wang Labortory Boston University Rutao Cui Laboratory University of Toronto Housheng He Laboratory Chicago University Dezheng Huo Laboratory Looking forward to comments and collaborations wangxi@tmu.edu.cn Wang