beta4 integrin.pptVIP

3.The β4 substrate domain promotes bFGF- and VEGF-mediated angiogenesis Matrigel plug assay:to examine if α6β4 signaling plays a role in bFGF-induced angiogenesis, Matrigel plugs containing bFGF were implanted in wild-type and mutant mice and recovered 7 days later. The β4 substrate domain promotes angiogenesis in response to bFGF in the Matrigel plug assay A:Macroscopic analysis revealed that the plugs from mutant mice were much paler than those from control mice . B: Confocal images of representative bFGF-containing plugs excised from FITC-Lectin-injected wild-type and mutant mice. C: bFGF-containing plugs from FITC-Lectin-injected wild-type and mutant mice were lysed and subjected to fluorimetry. the retinal neovascularization model:examine if α6β4 signaling plays a role in VEGF-induced angiogenesis E: P7 wild-type (WT) and mutant (1355T) mice were exposed to hyperoxia and returned to normoxic conditions. Eye cross-sections were stained with anti-PECAM-1 and counterstained with hematoxylin F: Quantification of vascular glomeruli abutting the limiting membrane in wild-type (WT) and mutant (1355T) retinas (n = 5 mice per genotype) 4.α6β4 signaling is not required for endothelial proliferation or survival ?The β4 substrate domain promotes branching of quiescent small-size vessels into proliferative microvessels without exerting a direct effect on endothelial cell proliferation A: Sections of bFGF-containing plugs from wild-type and mutant mice were stained with rat anti-PECAM-1 (left panels). Sections of bFGF-containing plugs from wild-type mice were subjected to double staining with goat anti-PECAM-1 (green) and rat anti-β4 (red) (right panels). The margins of the plugs are marked with a dotted white line. The endothelial cells of small vessels express α6β4 while those of capillaries do not. B: Sections of bFGF-containing plugs from wild-type and mutant mice injected with BrdU were subjected to double staining with anti-PECAM-1 (red) and anti-BrdU (green) (left