02细菌生理概要.ppt

Spread-plate method Sample is pipetted onto surface of agar plate (0.1 ml or less) Sample is spread evenly over surface of agar using sterile glass spreader Pour-plate method Typical spread-plate results Surface colonies Incubation Surface colonies Solidification and incubation Subsurface colonies Typical pour-plate results Sterile medium is added and mixed well with inoculum Sample is pipetted into sterile plate 固体培养基 -- 分离 Sample to be counted 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml 9-ml broth Total dilution Plate 1-ml samples 1/10 (10–1) 1/100 (10–2) 1/103 (10–3) 1/104 (10–4) 1/105 (10–5) 1/106 (10–