Bs8093菌株的鑒定及其对青枯雷尔氏菌的致弱作用.docVIP

Bs-8093菌株的鉴定及其对青枯雷尔氏菌的致弱作用? ?基金项目：国家863计划项目（2006AA10A211）；福建省发改委重大专项（闽农产（2006）10号） 作者简介：周先治（1976-），博士研究生，助理研究员。 *通讯作者：E-mail：laeptb@ 周先治1，2，张青文2，林营志1，朱育菁1，蓝江林1，刘波1* （1.福建省农业科学院生物技术研究所， 福州 350003；2.中国农业大学农学与生物技术学院，北京 100094） 摘要：通过形态特征、生理生化特征、脂肪酸分析和16S rDNA序列分析分离于番茄田土壤中分离的Bs-8093菌株进行了鉴定。结果表明，该菌株为球形芽孢杆菌，最适宜生长温度为30-35℃。研究发现，Bs-8093菌株对17株强致病力青枯雷尔氏菌的致弱能力不同，聚类分析将17株菌分为3类，第一类不易致弱菌株，包括菌株Rs-T.1.3-010702-01、Rs-T.1.3-010702-25、Rs-T.1.3-010702-27和Rs-E.1.3-010719-03；第二类菌株易致弱，包括菌株Rs-T.1.3-010702-07、Rs-T.1.3-010702-14、Rs-T.1.3-010702-17、Rs-T.1.3-010702-18、Rs-T.1.3-010702-19、Rs-T.1.3-010702-20、Rs-T.1.3-010702-21、Rs-T.1.2-010618-07、Rs-C.1.4-010704-02、Rs-G.1.4-010704-01、Rs-G.1.4-010704 关键词：Bs-8093菌株，鉴定，致弱作用 Identification of Strain Bs-8093 and Its Attenuation Effects on Virulent Strains of Ralstonia solanacearum Zhou Xian-zhi，Zhang Qing-wen，Lin Ying-zhi，Zhu Yu-jing，Lan Jiang-lin，Liu Bo (1. Biotechnology Research institute，Fujian Academy of Agricultural Sciences，Fuzhou 350003，China；2. college of Agronomy and Biotecnology，China Agricultural University，Beijing 100094，China) Absract:The bacterium strain Bs-8093 isolated from the soil of tomato field. This strain was identified as Bacillus sphaericus according to its morphological, physiological and biochemical characteristics, Fatty acid chromatogram and 16S rDNA sequence analysis. The optimum temperature for the growth of the strain was 30-35℃. Bs-8093 has attenuation effects on virulent strain of R. solanacearum, according to the difference attenuation ability of strain Bs-8093 to 17strains R. solanacearum, the 17strains divided into 3 groups, the first group is difficult to attenuate, including strain Rs-T.1.3-010702-01, Rs-T.1.3-010702-25, Rs-T.1.3-010702-27, Rs-E.1.3-010719-03. The second group is easy to attenuate, including strain Rs-T.1.3-010702-07, Rs-T.1.3-010702-14, Rs-T.1.3-010702-17, Rs-T.1.3-010702-18, Rs-T.1.3-010702-19, Rs-T.1.3-010702-20, Rs-T.1.3-010702-21, Rs-T.1.2-010618-07, Rs-C.1.4-010704-02, Rs-G.1.4-010704-01, Rs-G