免疫層析试纸划液仪的研制.docVIP

免疫层析试纸划液仪的研制 王清，郭雅婕 (河北工业大学控制科学与工程学院，天津 300130) 摘要：针对免疫层析检测试纸NC膜进行划液的专业设备，主要用于在表面划 关键词：免疫层析；划液仪；RS485总线；传感器；触摸屏 中图分类号：TP273 +.5 文献标志码：A Design and Implementation of Dispenser Device For immunochromatographic strip WANG Qing, GUO Ya-jie (School of Control Science and Engineering, Hebei University of Technology, Tianjin 300130,China) Abstract: According to the shortage of the production of immunochromatographic strip C/T line, It designed a professional draw liquid equipment for a set of constant linear velocity movement of NC membrane, mainly used to draw quantitative protein solution in the strip of NC film surface. In order to meet the requirements, dispenser instrument used single chip microcomputer as main control core, manual adjusted the position platform marking the appropriate location and spacing. Designed the position platform of independently regulate and precise and stable gearing, through the keyboard and LCD display made operation more simple and intuitive, liquid detection device increased the precision and stability. Add the RS-485 communication interface, so PC could be easy to control the instrument through the Modbus protocol when it used in the network. Practice proves, the performance of the instrument is economical and simple, performance stable and operate accuracy. It can be applied to the research and the development of small and medium-sized enterprises. Key words: immunochromatographic; dispenser instrument; SCM; gearing; step motor 0引言 近年来免疫学快速诊断和检测方法广泛应用各个领域。胶体金免疫层析技术凭借其快速、简便、精确、稳定等优点，已经成为近年来生物产业化最成功的产品之一。做为免疫纸生产的专业设备，主要就是用于在NC膜表面划出C/T线，鉴于检测试纸的广泛应用，研究与开发具有广阔的市场和应用前景。 国内生产的设备远不及国外将抗体划到膜表面，膜本身的物理性质为软脆，在其表面留下印痕进口的由于使用的材料和控制系统较好，所以留下的划痕较轻，而国产仪器较差，留下的划痕也就比较严重。划痕容易对层析的金标复合物形成阻力，导致假阳性。同时跑板时在T线位置出现若有若无一条细线（鬼线），而跑板结束后鬼线消失的奇怪现象。针对这一现象，开发一套控制的显得尤为重要。 仪器主要参数如下： 总量：； 背景量(划液速度)：； 行程控制精度：（满行程）； 通讯接口：RS485 电源：DC24.0V； 工作温湿度 ：5~40℃，30%~75 %RH； 储存温湿度 ：-10~60℃，10% ~93% RH； 大气压力：700~1060hpa； 划膜装置配用注射器规格：1mL\3mL\5mL\10mL\20mL\30mL\60mL。 划液仪需满足对