缺硫脅迫对水稻叶片超微结构的影响-yzxzcom.docVIP

硫素营养胁迫对水稻根系和叶片超微结构的影响 张秋芳1，彭嘉桂2，林 琼2，杨 杰2，曹志洪3 （1福建省农业科学院生物技术研究所生物农药研究中心，福建 福州 350003； 2福建省农科院土壤肥料研究所，福建 福州 350013, 3中国科学院南京土壤研究所，江苏 南京 210008） 摘 要：硫是作物必需的营养元素之一，作物硫素营养胁迫会诱导产生某些异常的代谢活动，严重影响其生长发育。本研究通过水培试验，用电镜技术了解不同浓度硫素营养对水稻根系和叶片超微结构的影响。结果表明，受硫素营养胁迫的水稻，其根系及叶片的超微结构均发生了显著的变化：低硫胁迫会造成水稻叶片叶肉细胞内的叶绿体结构肿胀，基粒片层松驰、散乱，细胞器减少，线粒体结构被破坏，根系细胞内几乎没有内含物；硫浓度高则会使叶片叶绿体基粒片层致密、浓缩，无规则化，根系细胞发生质壁分离。这就从微观上进一步解释了水稻硫素营养胁迫严重影响其生长发育的原因与机理，也为水稻硫素营养的早期诊断提供了理论依据。 关键词：硫素营养；胁迫；水稻；根系；叶片；超微结构 Effect of sulfur stress on ultrastructure of rice in roots and leaves Zhang Qiu-fang1 , Peng Jia-gui2, Ling Qiong2 and Yang Jie2,Cao Zhi-Hong (1 Biocide Center Biotechnology Institute, Fujian Academy of Agricultural Sciences, 2 Soil and Fertilizer Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 3Institute of Soil Science,Chinese Academy of Sciences,Jiangsu,Nanjing Abstract：Sulfur is one of the essential nutrition elements of crops. S-nutrition stress of crops can induce some abnormal metabolizable activities and prevent from growing seriously.The ultrastructures of leaves and roots with variouse sulfur concentration of rice grown in water culture were studied by using TEM. The results indicated that the ultrastructures of leaves and roots of rice were different significantly with various available-S levels. The main results show: To the plant of S-deficient treatment , organella in the leaves stigmatic cells were hypoplasia and destroyed,deformed.Grana lamella of chloroplast were in loosing and disorder .What’s more ,there is almost no thing in the root cell.To the plant of S-excessive treatment, Grana lamella of chloroplast were heavily thick, concentrative,continue but not broken.In the root cells,plasma membrane walls desperated from cell wall,the plasma were thicker. Therefore,it explains the reason and mechanism of the influence of S-stress on the growth of rice,and provides the theory evidences of diagnoses of S nutrition of rice early. Key words： Sulphur nutrition; Stress; Rice; Root; Leaf