TLC与HLC测定柚皮中柚皮苷和橙皮苷.docVIP

TLC与HPLC测定柚皮中柚皮苷和橙皮苷625014；2.达州市质检中心，四川达州 635000) 摘要 目的：建立柚皮中柚皮苷、橙皮苷的薄层色谱和高效液相色谱同时测定方法。方法：薄层色谱法以氯仿∶甲醇∶=11∶3∶1为展开剂，聚酰胺为吸附剂的薄膜立式上行展开；液相色谱法采用Luna C18 (2)柱（4.60 mm×150 mm，5 μm，100 ?）色谱柱；水∶乙腈（81∶19）为流动相，磷酸调pH为2.4；检测波长为283nm；流速为1.0 mLmin；柱温为30 ℃。结果：中柚皮苷和橙皮苷得到分离，Rf分别为0.4和0.69；中柚皮苷橙皮苷柚皮苷在0.020.99 μg范围内有良好线性关系，r=0.9999；橙皮苷在0.0281.1 μg的范围内有良好线性关系，r=0.9999。柚皮中柚皮苷含量分别是29.90 mg、28.39 mg和24.56 mg，橙皮苷薄层色谱检出结论：本方法可同时测定柚皮中柚皮苷和橙皮苷的含量。 关键词柚皮苷；橙皮苷；薄层色谱；高效液相色谱HPLC Determination of the Contents of Naringin and Hesperidin in pummelo pericarp Abstract Objective: To establish an optimum TLC and HPLC methods to determination of naringin and hesperidin in pummelo pericarp simultaneously. Methods: The chromatogram condition of TLC was follows: the developing agent was chloroform and methanol and acetone (11:3:1), the polyamide was the absorbent and developing with vertical type. Naringin and hesperidin were determined by HPLC on Luna C18 (2) column (4.60 mm×150 mm, 5 μm, 100 ?) at 30 ℃, the mobile phase was pure water ? acetonitrile (81:19), adjusted to pH 2.4 with phosphoric acid. The UV detection wavelength was set at 283 nm. The flow rate was 1.0 mL/min. Results: Naringin and hesperidin were separated in TLC by the Rf were 0.49 and 0.69, respectively. The separation was higher than 2.0 between naringin and hesperidin, the column plates both of the naringin and hesperidin were not less than 8000 on the conditions of HPLC; The calibration range of naringin and hesperidin were 0.025-0.99μg (r=0.9999) and 0.028-1.1μg (r=0.9999), respectively. The contents of naringin of 3 varietal pummelo pericarps were 29.90 mg/g, 28.39 mg/g and 24.56 mg/g, respectively. And hesperidin in 3 varietal pummelo pericarps was only detected by TLC. Conclusions: The method was convenient and accurate of simultaneous determination of naringin and hesperidin contents in pummelo pericarp. Key words Pummelo pericarp; Naringin; Hesperidin; TLC; HPLC 柚是芸香科柑桔属Citrus L.植物，又名朱栾、雪柚气柑。主要的黄烷酮，橙皮苷是橙皮素与芸香糖形成的糖苷，为二氢黄酮衍生物。