膀胱移行细胞癌组织MVD和-连环素蛋白表达及其相关性研究.docVIP

膀胱移行细胞癌组织MVD和?-连环素蛋白表达 及其相关性研究 李乐山1，王化修2 （1.邵阳市中心医院泌尿外科；2.邵阳医学高等专科学校病理教研室，湖南 邵阳422000MVD）和?-catenin蛋白在膀胱移行细胞癌中的表达及临床意义。方法 采用免疫组化SP法检测10例正常膀胱黏膜和52例膀胱移行细胞癌组织中CD105标记的MVD及?-catenin蛋白表达。结果 10例正常膀胱黏膜和52例膀胱移行细胞癌组织中CD105标记的MVD值分别为1.78±1.28和19.22±10.16；?-cateninI、II、III级膀胱移行细胞癌中的异常表达率分别为50.0%(10/20)、80.9%(17/21)、100.0%(11/11)，膀胱移行细胞癌浸润组中?-catenin蛋白异常表达率（90.6%，29/32））β-catenin异常表达的癌组织中MVD值显著高于正常表达者。结论 MVD是影响膀胱移行细胞癌预后的因素之一，β-catenin可能在膀胱移行细胞癌组织微血管形成中发挥作用。 关键词： 膀胱肿瘤；癌，移行细胞；微血管密度；?-连环素; CD105 The Relationship between Microvessel Density and the Expression of β-catenin Protein in Bladder Transitional Cell Carcinoma LI Le-shan1,.WANG Hua-xiu2. Department of Urology,Shaoyang Center Hospital,Shaoyang,422000,China; 2. Department of Pathology,Shaoyang Medical College, Shaoyang,422000,China) Abstract:【Objective】To investigate the correlation between microvessel density(MVD) and expression of β-catenin protein in bladder transitional cell carcinoma(BTCC) and its clinical significance. 【Methods】The expression of β-catenin protein and CD105 were measured by immunohistochemistry in 52 cases of BTCC tissues and 10 normal bladder tissues and average MVD was assessed using CD105 antibody.【Results】The average MVD in 10 normal bladder 【基金项目：湖南省（2007]256号） tissues and 52 cases of BTCC tissues was 1.78±1.28 and 19.22±10.16,respectively.The abnormal expression rate of β-catenin protein was 50.0%(10/20),80.9%(17/21),100.0%(11/11) in G1,G2,G3 of BTCC.The abnormal expression rate of β-catenin protein in group Ta~T1 was higher than that in group T2~T3.The MVD in the tissues with abnormal β-catenin expression was significantly higher than the controls.【Conclusion】MVD is a prognostic factor, β-catenin might promote angiogenesis in BTCC Tissues. Key words: Bladder neoplasma;Transitional cell carcinoma;Microvessel density; β-catenin; CD105 在肿瘤分子生物学领域Wnt信号通路是研究的热点之一, Wnt信号通路的异常会导致细胞的恶性转化, Wnt信号转导途径的核心机制是游离的β-连环素(β-catenin)在细胞内蓄积,