lysine624oftheamyloidprecursorprotein(app)isacritical.docxVIP

Lysine 624 of the Amyloid Precursor Protein (APP) is a Critical Determinant of Amyloid Beta Peptide LengthSupplemental DataSupplemental Figure 1. Mutation of Lysine 28 does not produce a corresponding shift in Epsilon-cleavage of AICD. Membrane fractions were isolated from HEK 293T cells overexpressing C99-FLAG wt and C99-FLAG K28A and incubated at pH 6.8 for two hours to induce γ-secretase activity. AICD fragments were immunoprecipitated from samples with anti-FLAG (M2) beads and analyzed by MALDI-MS. AICD 50-99 FLAG was the major species produced followed by 49-99-FLAG and 48-99-FLAG for b