高效液相色测定米糠中水溶性维生素.docVIP

高效液相色谱法测定米糠中的水溶性维生素摘要：高效液相色谱法同时测定米糠中四种水溶性维生素VB1，VB6烟酸，烟酰胺的含量。在Hypersil C18 （250 cm×4.6 mm i.d., 5.0 μm）上，以体积比为88:12的0.1％磷酸水溶液-甲醇为流动相，以0.6 mL/min流速等度洗脱，在266nm波长下检测。各组分在8min能基线分离。四种维生素在0.6g/mL～85×10-5g/mL范围内呈线性关系，VB1、VB6、烟酸和烟酰胺的检出下限分别为0.43 g/mL、0.34g/mL、0.32 g/mL、0.30 g/mL。经简单预处理，可以对米糠和淘米水中的这四种维生素的含量进行测定，平均回收率在96.0％～99.9％。该法简便、快速、准确、灵敏，对复杂体系水溶性维生素的测定具有重要的参考价值。 关键词：水溶性维生素；米糠；高效液相色谱water-soluble vitamins in rice bran by reverse-phase high performance liquid chromatography CAO Rui, CAO Yu-hua, LI Nan, GONG Wen-jun, KONG Ling-yao, WANG Yun School of Chemical and Material Engineering, Southern Yangtze University, Wuxi 214122 Abstract: High performance liquid chromatography (HPLC) has been applied for the simultaneous determination of four kinds of water-soluble vitamins including vitamin B1, vitamin B6, nicotinic acid, and nicotinamide in rice bran. The separation was performed on a Hypersil C18 column (250 cm×4.6mm i.d., 5.0 μm) with a mobile phase of 0.1% H3PO4- methanol (88:12, V/V) at flow rate of 0.6 mL /min, and detection wavelength was 266 nm. Four vitamins had good linearity in the range of 6.0×10-7g/mL - 8.5×10-5g/mL, with detection limit of 4.3×10-7 g/mL, 3.4×10-6g/mL, 3.2×10-7g/mL and 3.0×10-7 mg/L for vitamin B1, vitamin B6, nicotinic acid and nicotinamide, respectively. Baseline separation of four vitamins can be achieved within 8 minutes. Without complicated pretreatment, four analytes in rice bran samples, as well as extracts from rice with water, were successfully determined with recoveries between 96.0% and 99.9%. The approach could be applied to determine water-soluble vitamins in complicated samples owing to simplicity, convenience, sensitivity and accuracy. Key words: water-soluble vitamins; rice bran; high performance liquid chromatography 米糠是一类具有广泛开发潜力的高附加值资源，含有稻米的64％营养物质及90％人体所需要各种营养元素[1]，包括维生素、氨基酸、脂肪酸、矿物质，还有米糠多糖、二十八碳烷醇、神经酰胺等生理功能卓越的活性物质[2]。其中水溶性维生素是一类重要的营养成分，在各种保健品[]，功能型饮料中烟酸和烟酰胺能够促进头皮血液循环，防止脱发；VB6具有美发功效；VB1具有滋养与深层清洁的作用，因此化妆品中添加微量的维生素。淘米水中也含有维生素