MycobacteriumsmegmatisKubindsDNAwithoutfreeends.pptVIP

Mycobacterium smegmatis Ku binds DNA without free ends by Ambuj?K. Kushwaha, and Anne Grove Biochem. J. Volume 456(2):275-282 December 1, 2013 ?2013 by Portland Press Ltd TKu can bind internal DNA sites (A) Electrophoretic analysis of 50?bp DNA titrated with TKu. Lane 1, 50?bp DNA (0.5?nM) only; lanes 2–15, DNA titrated with increasing concentrations (0.2–80?nM) of TKu. (B) C-terminal sequence of M. smegmatis Ku protein with... Ambuj?K. Kushwaha, and Anne Grove Biochem. J. 2013;456:275-282 ?2013 by Portland Press Ltd TKu protects DNA from exonucleolytic cleavage Lane 1, 50?ng of 1?kb DNA only;