HCV非结构蛋白3反式激活基因6转染肝癌细胞的基因表达谱芯片分析.docVIP

HCV非结构蛋白3反式激活基因6转染肝癌细胞的基因表达谱芯片分析 作者：邵清，成军，王琳，张健，卢成哲 【关键词】 丙型肝炎病毒 Screening of genes differentially expressed in HepG2 cells transfected with HCV NS3TP6 by microarray assay 【Abstract】 AIM: To study the difference in gene expression in human hepatoblastoma cell line HepG2 cells transfected with HCV NS3TP6expressing plasmid and to further elucidate its molecular biological mechanism. METHODS: Sequence specific primers were designed and synthesized and the NS3TP6coding DNA fragment was amplified with polymerase chain reaction techniq