HtrA基因缺陷对肺炎链球菌毒力影响的研究.docVIP

HtrA基因缺陷对肺炎链球菌毒力影响的研究 作者：黄远帅，许颂霄，朱旦，阳强，刘明芳，陈淑惠，张雪梅，尹一兵 【关键词】 肺炎链球菌；毒力因子；密度感应系统；HtrA基因 comEffect of HtrA gene deletion on virulence of Streptococcus pneumoniae 【Abstract】 AIM: To investigate the effect of HtrA gene deletion on the virulence of Streptococcus pneumoniae. METHODS: HtrAdeficient strain was constructed by insertion, replication and inactivation, and identified by PCR. Relative quantitative RTPCR was used to measure the expression levels of virulent factors and study the virulence change by mice virulence experiments. RE