licD2基因缺陷导致肺炎链球菌毒力下降.docVIP

licD2基因缺陷导致肺炎链球菌毒力下降 作者：蓝锴，单幼兰，袁军，孟江萍，张雪梅，尹一兵 【关键词】 缺陷 Deletion of licD2 gene leads to reduced virulence of Streptococcus pneumoniae 【Abstract】AIM: To assess the role of licD2 gene in the virulence of Streptococcus pneumoniae. METHODS: licD2deficient strain was constructed and identified by PCR. Relative quantitative RTPCR was applied to measure the expression levels of virulent factors and the virulence was studied by mice survival test. RESULTS: The expression of virulent factors in licD2deficient strain was lower than that