Survivin基因靶向RNA干扰重组表达载体的构建和测序.docVIP

Survivin基因靶向RNA干扰重组表达载体的构建和测序 【关键词】 Survivin Cloning and sequencing of recombinant plasmid affecting survivin gene translation by RNA interference technique 【Abstract】 AIM: To clone the recombinant plasmid affecting survivin gene translation by RNA interference and to analyze the nucleic acid sequence of the recombinant plasmid for new gene therapy for tumors. METHODS: Two DNA sequences containing small hairpin structure were designed and synthesized. The complement form was obtained by annealing and being cloned into vector pSile