人肥大细胞羧基肽酶的单抗2A9识别表位的预测和鉴定.docVIP

人肥大细胞羧基肽酶的单抗2A9识别表位的预测和鉴定 作者：周艳春，侯一峰，陈霖霏，郑晓璇，何韶衡 【关键词】 肥大细胞羧基肽酶；表位,B淋巴细胞；预测；基因表达 【Abstract】 AIM: To predict the B cell epitope of human mast cell carboxypeptidase and identify the region of B cell epitope recognized by monoclonal antibody against hMCCP. METHODS: The B cell epitopes were predicted by secondary structure , hydrophilicity, antigenic index and surface probability plot . The cDNA encoding the N terminal 1～111, 97～202 and 1～202Aa of hMCCP was amplified by PCR and cloned into prokaryotic expression vector pET44 Ek/LIC. Aft