瓠子籽中脂酸成分的GC.docVIP

瓠子籽中脂肪酸成分的GC/MS分析 摘要 瓠子是夏秋季供应的主要蔬菜之一，其果肉白而柔嫩，水分和纤维少，品质佳近，也是一种重要的经济作物。本实验采用索氏提取法对瓠子种子中的脂肪酸进行了提取，用氢氧化钾-甲醇法酯化后，以气相色谱-质谱联用仪进行了分析。分离鉴定出五种脂肪酸，并对其主要成分进行了鉴定，结果表明：瓠子籽中主要化学成分及含量为：十六烷酸(6.46%)、十八烷酸(3.89%)、蓖麻油酸(0.69%)、11-二十碳烯酸(0.83%)和9,12-十八碳二烯酸(87.81%)。并且脂肪酸的提取率为39.67%。为进一步开发研究瓠子资源提供了科学依据。 关键词：瓠子种子；脂肪酸；索氏提取；气相色谱/质谱 ANALYSIS OF FATTY ACIDS IN PERICARP OF HISPID BOTTLE GOURD SEEDS BY GC/MS ABSTRACT Pericarp of Hispid Bottle Gourd is one of the main summer supply vegetables, the pulp white and tender, water and piper less, the quality is good close, and also is a kind of important economic crops. In this paper, The Sox let extraction, Gas chromatography mass spectrometries were analyzed separately. Through this experimental study, Mastering the Sox let extraction method for the determination of the principles and methods of operation of the crude fat content. The results indicated that the seed of Pericarp of Hispid Bottle Gourd contained five kinds of fatty acids: hexadecane acid was6.64%,octadecanoic acid was3.89%,Methyl ricinoleate acid was 0.69%,11-Eicosenoic acid was0.83%,9,12-Octadecadienoic acid was 86.22%. The total fatty acid extraction yield is 39.67%.For further development research Pericarp of Hispid Bottle Gourd resources and offer scientific basis. Key Words: Pericarp of Hispid Bottle Gourd seed; Fatty acid; Soxhlet Extraction; GC/MS 目录 1 前言-------------------------------------------------------------------------------------------1 1.1瓠子简介-----------------------------------------------------------------------------------1 1.2 脂肪酸简介-------------------------------------------------------------------------------2 1.2.1饱和脂肪酸-------------------------------------------------------------------------2 1.2.2单不饱和脂肪酸-------------------------------------------------------------------2 1.2.3多不饱和脂肪酸------------------------------------------------------------------3 1.3油脂提取方法---------------------------------------------------------------------------- 5 1.3.1油脂的传统提取方法-----