Capillary Columns Seton Hall University Pirate Server毛细管柱西东大学海盗服务器.ppt

Capillary Columns Nicholas H. Snow Department of Chemistry, Seton Hall University, 400 South Orange Avenue, South Orange, NJ 07079 snownich@shu.edu Capillary/Open Tubular Column WCOT - Wall Coated Open Tubular Capillary Column Dimensions Other Types of Capillary Columns Tubing Material Stainless Steel reactive Glass can be made inert difficult to handle Fused Silica flexible most inert most popular Fused Silica Surface Contains 0.1% -OH groups Very inert Uniform chemical surface Fused Silica High tensile strength Flexible Sheath of polyimide Very inert Capillary vs. Packed Packed Column - ECD Capillary Column - ECD Important Column Parameters Column Diameter Column Length Column Length Recommendations Stationary Phase Film Thickness Starting point: 0.25mm Compromise: resolution and capacity Practical operating temperatures Speed or resolution Thick Film Stationary Phase Advantages increased retention for volatiles increased capacity Disadvantages less efficient higher temperatures higher bleed Natural Gas Thin Film Stationary Phase Advantages high efficiency lower elution temperatures fast analysis Disadvantages low capacity limited trace analysis Thin Film SeparationAir Freshener Stationary Phase Requirements Selectivity Low Bleed Rate Reproducibility Common Stationary Phasespolysiloxane gum phases Stationary Phasespolyglycol Crosslinked Stationary Phases More stable Clean by rinsing Longer lifetimes Carrier Gas Flow Rate Review - Capillary Columns Review - Capillary Columns Column Selection * * * *