骨保护素对破骨细胞的影响程度分析.docVIP

骨保护素对破骨细胞的影响程度分析 [摘要] 目的 通过分析比较不同浓度的骨保护素（osteoprotegerin，OPG）对破骨细胞（osteoclasts，OC）活性的影响程度来研究2者在生理活性及生理功能之间的相关性。方法 本次实验应用6周龄的雌性小鼠模型，添加不同浓度的骨保护素后通过抗酒石酸酸性磷酸酶（tartrate resistant acid phosphatase，TRAP）染色、细胞骨架F-actin染色及骨吸收陷窝的检测，观察OPG与OC之间的相关性。结果 OC培养1h内即贴壁生长，并为展开且无大的多核OC存在。培养至第5天，较小的单核细胞逐渐开始相互融合，形成多核细胞且特征明显。OPG处理3 d后，对照组细胞形态整体性良好，利用观察比较。随着OPG 浓度的增大，实验组的多核细胞数逐渐减少，多核细胞数随着骨保护素（OPG）浓度的逐渐增加而减少，2者间呈现负相关的关系（r=0.516，P＜0.05）。且当骨保护素（OPG）浓度为50μg/L 时，视野内仅存在少量多核细胞，模糊可见。培养结束后，OPG组多核细胞数随着OPG浓度的增大而逐渐减少，且OPG浓度为50ng/mL时仅有少量多核细胞可见；OPG的浓度为20、50 μg/L时，OPG组的OC数较对照组显著减少（P0.05），而当OPG的浓度为100μg/L时，OPG组的OC数较对照组呈极显著减少（P0.01）；OPG实验组的骨吸收陷窝的面积、密度和减弱程度均与OPG的浓度呈正相关（r=0.459，P0.05），且面积比较具有显著性差异（P0.05）。结论 不同浓度的OPG对破骨细胞的影响程度不同，且OPG的浓度越大其抑制性越强。 [关键词] 骨保护素；破骨细胞；吸收陷窝 [中图分类号] R3 [文献标识码] A Analysis of?the influence degree of?osteoprotegerin?on?osteoclasts LIU Jian1，SONG Hui2 (1.Department of orthopedics,?Shandong Hospital?of Shengli oil field?in Dongying City,Dongying 257000,China.2. Department of oral and maxillofacial surgery,?Shandong University Jinan 250000，China.) [Abstract]: Objective Effects of different concentrations of OPG (osteoprotegerin, OPG) on osteoclasts (osteoclasts, OC) the degree of influence activity, provide a theoretical basis for further research OPG and physiological functions of OC. Methods 6-week-old female mice were cultured in vitro on the basis of adding different levels of OPG, by TRAP staining, F-actin cytoskeleton staining and resorption correlation detection nest was observed between OPG and OC. Results OC culture that is adherent growth within 1h, and for the launch of a large multi-core and no CO present. Cultured for 5 days, the smaller monocytes gradually began to fusion with each other to form multinucleated cells and distinctive characteristics. OPG treatment after 3 d, cell morphology control group overall good use of observation and comparison. With OPG concentration increases, the number of cells in the experimental group multicore gradually reduce the number of