硕士论文开题报告模板..docVIP

硕士学位论文开题报告 （硕士学位论文题目：宋体，小二号字，加粗） 研 究 生： 欧阳路平 指导教师： 尹淑霞 学 号： 3127897 学 院： 林学院 专 业： 草学 北京林业大学研究生院 二〇一三年三月 一、摘要 论文题目 中文 草地早熟禾在干旱和褐斑病胁迫下MicroRNA的表达分析 英文 The Expression Analysis of MicroRNA in Kentucky Blue Grass with brown blotch disease and drought stress 摘 要（不超过800字） 小RNA（即MicroRNA或miRNA）是一类新发现的广泛存在于真核生物中， 非编码的单链 RNA 短序列， 一般长度为20~24 nt。一些研究表明，MicroRNA在植物受到胁迫过程中，扮演了十分重要的角色。草地早熟禾是非常重要的草坪草之一，在世界范围类应用也是最为广泛的。而草地早熟禾又极易感染褐斑病。褐斑病(Rhizoctonia solani)又称立枯丝核疫病, 是报道最早的、危害最为严重的草坪草病害之一。作为一种土传病害, 褐斑病具有病原物复杂、发病率高、传染迅速、危害严重等特点。本实验，主要通过对草地早熟禾进行干旱处理和接种褐斑病，来研究其在非生物胁迫和生物胁迫条件下，根和叶片中MicroRNA表达的差异。从而摸索出MicroRNA在草地早熟禾受到胁迫时的生理作用。因此，开展相关研究不仅对于草地早熟禾褐斑病的防治具有重要的实践意义。而且，对草坪草进行耐干旱机制的研究，在节水越来越重要的当今社会也显得十分重要了。由于目前几乎还没有关于草地早熟禾在胁迫条件下MicroRNA表达的研究，所以本研究还为以后有关草坪草MicroRNA的研究提出了理论依据。 Small RNA ( MicroRNA or miRNA) are a class of endogenously non-coding RNAs that are recently found widespread in eukaryotes, generally of 20-24 nt.Some studies have shown that MicroRNA plaly a very important role in the process when the plants are offered from stress.Kentucky bluegrass is one of the very important turfgrass that is widely used in the world .However,Kentucky bluegrass is to be infected by Brown spot which also called Rhizoctonia blight.It is the earliest reported and one of the most serious hazards turfgrass diseases.As a soil-borne diseases, Brown spot owes the features of complex pathogens , high incidence , spread quickly and serious harm.This study is mainly to explore the physiological role of MicroRNAs when the Kentucky bluegrass suffers from some stresses,such as drought and diseases.We extract RNAs from the leaves and roots after the grasses are treated by stresses.And then ,analysis the differences of MicroRNA” expression. Therefore, it has important practical significance for the prevention and treatment of Kentucky bluegrass Brown spot to carry out related res