protocol for immunization.doc

Immunization and serum-collection procedures for producing custom rabbit antibodies. We offer three standard protocol packages and several extension options (immunization and serum collection schedules) for rabbit polyclonal antibody production within our complete array of Thermo Scientific Pierce Custom Antibody Services. Highlights: Custom rabbit antibodies - three standard protocol options for immunizing rabbits, collecting serum, testing bleeds, reporting titer results and delivering antisera Rabbit titer guarantee - if you allow us to oversee design and production of the peptide and/or protein antigen used for injection, we guarantee to reach a minimum rabbit antiserum titer of 1:10,000 as measured with our ELISA assay Track and customize project - easily add additional rabbits to the default protocol; track and manage projects online with OpenProject Tool: you decide which animals to continue or terminate (see Extensions below) Full service integration - combine this immunization service with our complete set of services for antigen preparation and antibody purification (see Home or Custom Polyclonal Antibodies pages for details) Rabbit Immunization Protocols 2 Rabbit, 70 Day Protocol: Of the three standard options for rabbit polyclonal antibody production, this protocol is optimized to produce antiserum in the shortest possible time. It is the most affordable option, popular among individual academic researchers. The default immunization schedule uses two NZW SPF rabbits (New Zealand White rabbits that are Specific Pathogen-Free); additional rabbits are easily added to the standard order for a modest cost. Injections are subcutaneous (SQ) as emulsions in Complete Freunds Adjuvant (CFA) or Incomplete Freunds Adjuvant (IFA); alternative adjuvants can be used if requested. Continuation of rabbits after Day 60 is offered on a month-to-month basis (see Protocol Extensions below). Standard 70-Day Rabbit immunization protocol for rabbit polyclonal antibody productio