移液器的使用学案.pptVIP

移液器工作原理： 移液器的维护保养 正确的吸头安装方法 常见的错误安装方法 在将吸头套上移液器时，使劲地在吸头盒上敲几下。 可能导致地后果 1.移液器的内部配件（如弹簧）因敲击产生的瞬时撞击力而变得松散，甚至会导致刻度调节旋钮卡住 。 2.移液器套柄弯曲 正确的吸头安装方法: 单道移液器：移液器垂直插入吸头中，稍微用力左右微微转动即可使其紧密结合 如何防止液体进入套柄 利用带滤芯的吸头，它有内部疏水过滤器，避免气雾和液体进入套柄。 当吸头中有液体时，不要颠倒或平放移液器。 缓慢吸液，握住移液器保持垂直或20度以内倾角。 量程在5ml和5ml以上的移液器，配合使用厂家提供的专用安全过滤器。 移液器正确的放置方法 1.如不使用，要把移液器的量程调至最大值的刻度，使弹簧处于松弛状态 以保护弹簧 2.使用完毕，尽量将其竖直挂在移液枪架或挂钩上，但要小心别掉下来。这种保管方式可以延长移液器的使用寿命. 定期测漏和校准 定期检查是否有漏液现象。简易的方法如下： 吸取液体后将移液器垂直放置在枪架上，静止15秒，观察吸头顶端是否有液滴 如果漏液，原因大致有一下几方面：1、吸头是否匹配；2、密封系统是否正常；3、如果是易挥发的液体（许多有机溶剂都如此），则可能是饱和蒸汽压的问题。可以先吸放几次液体，然后再移液。 比较专业的方法是使用瑞宁公司的测漏仪。 由厂家或专业机构提供定期的较准，通常一年一到两次 高粘度样品移液的窍门: 反向模式移液 适用于高粘度样品 不适用于水溶液 同常规移液操作相反的流程 第二零点吸液 第一零点排液 敬请批评指正！ 5 Q: How big an error do you expect from individual pipetting-technique-errors? Q. How big do you expect an accumulated error to be? A. You‘ll be amazed…… Let‘s have a look. 6 Pipette Specifications Before we begin our review of specific pipetting techniques, I want to define pipette accuracy, as well as another important term – precision. Accuracy is the ability of a pipette to give a response close to a true or nominal volume as indicated by the volume setting. Typical accuracy specifications for air-displacement pipettes are approximately 1% for pipettes with nominal volume settings greater than 35%. For pipette volume settings less than 35%, the specifications can be as much as 3 times less accurate. Precision is a qualitative term and is the ability of the pipette to provide closely similar responses. A typical precision specification for air-displacement pipettes is about 1/3 to 1/4 of the accuracy specification. Precision is often referred to as repeatability or sample reproducibility, and also as standard deviation. Now that we’ve reviewed the pipetting cycle and defined and discussed terms such as accuracy and precision, I would like to discuss the basics of proper pipetting technique and review some recommended techniques to help you improve your pipettin