构建叶酸修饰的乳腺癌靶向纳米超声造影微粒.docVIP

构建叶酸修饰的乳腺癌靶向纳米超声造影微粒 陈园园1，徐 枫1，杨 辉2，刘 婷1，周建桥3，蔡晨蕾4，叶园园1，刘培峰4，韩宝三1(1上海交通大学医学院附属新华医院普外科、普外科实验室，上海市 200092；2教育部高分子合成与功能构造重点实验室，浙江大学高分子科学与工程学系，浙江省杭州市 310027；3上海交通大学医学院附属瑞金医院超声科，上海市 200025；4上海交通大学医学院附属仁济医院中心实验室，上海市 200127) 引用本文：陈园园，徐枫，杨辉，刘婷，周建桥，蔡晨蕾，叶园园，刘培峰，韩宝三. 构建叶酸修饰的乳腺癌靶向纳米超声造影微粒[J].中国组织工程研究，2016，20(30):4425-4433. DOI: 10.3969/j.issn.2095-4344.2016.30.003 ORCID: 0000-0002-0322-5403(韩宝三) 文章快速阅读： 文题释义： 纳米级超声对比剂：具有较强的穿透能力，能够穿越血管内皮进入组织间隙，使血管外靶组织显像成为可能，从而超越了微米级对比剂仅能发生血池内显像的局限性，推动了超声分子显像技术的发展，特别是针对肿瘤等重大疾病的早期诊断技术；使构建纳米级靶向超声对比剂成为国内外研究的热点。是利用对比剂表面固有的化学特性或通过对对比剂表面进行特殊处理，构建特异性靶向超声对比剂，使其经静脉注入后能靶向性聚集并较长时间滞留于靶组织器官，再通过超声对比显影，产生分子水平显影，提高超声对早期病变的诊断及鉴别能力。 Construction of folate-modified nanoparticles as ultrasound contrast agent targeting breast cancer Chen Yuan-yuan1, Xu Feng1, Yang Hui2, Liu Ting1, Zhou Jian-qiao3, Cai Chen-lei4, Ye Yuan-yuan1, Liu Pei-feng4, Han Bao-san1 (1Department of General Surgery, General Surgery Laboratory, Affiliated Xinhua Hospital, Medical School of Shanghai Jiao Tong University, Shanghai 200092, China; 2Key Laboratory of Macromolecular Synthesis and Functionalization of Ministry of Education, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang Province, China; 3Department of Ultrasound, Affiliated Ruijin Hospital, Medical School of Shanghai Jiao Tong University, Shanghai 200025, China; 4Medical Science Research Center, Affiliated Renji Hospital, Medical School of Shanghai Jiao Tong University, Shanghai 200127, China) Abstract BACKGROUND: Studies have testified that nano-ultrasound contrast agents have a strong permeability, making it possible to image the targeted tissues outside blood vessels and overcome the limitation that micron contrast agents are only available for the blood pool imaging. OBJECTIVE: To construct the folate-modified nanoparticles targeting breast cancer as ultrasound contrast agents, as well as to observe their ability to specifically bind to cells and ima