利用T细胞受体变异β基因谱系分析T细胞急性淋巴细胞白血病病人T细胞克隆性..docVIP

利用T细胞受体变异β基因谱系分析T细胞急性淋巴细胞白血病病人T细胞克隆性 中国病理生理杂志 2000年第7期第16卷 论著 作者：李杨秋　陈少华　杨力建　祁明芳 关键词：受体，抗原，T细胞；基因；白血病 　　［摘　要］　目的：分析T细胞-急性淋巴细胞白血病(T-ALL)病人的T细胞克隆性。方法：利用RT-PCR方法分析6例T-ALL和10例正常人外周血单个核细胞中24个T细胞受体变异β(TCR Vβ)基因的CDR3长度，PCR产物再进一步进行基因扫描和序列分析。结果：3例病人的某些TCR Vβ亚家族T细胞呈单克隆或寡克隆性增殖，主要为Vβ2、3、6、9、21和24。其它3例及正常人均表现为多克隆性增殖T细胞。结论：部分T-ALL来自于TCR Vβ亚家族克隆性增殖T细胞。该方法有助于临床上检测微小残留病变。 　　［中图分类号］　R733.71；R392.11　　　［文献标识码］　A Analysis of T cell clonality by using T-cell receptor varible β gene 　　repertoire in T-cell acute lymphoblastic leukemia· LI Yang-qiu, CHEN Shao-hua, YANG Li-jian, QI Ming-fang 　　(Department of Hematology, Medical College, Jinan Univesity, Guangzhou 510632， China) 　　［Abstract］　 AIM: To analyze T cell clonality in patients with T cell acute lymphoblastic leukemia (T-ALL). METHODS: The complementarity determining region 3 (CDR3) size of 24 T cell antigen receptor variable β (TCR Vβ) region gene was analyzed in peripheral blood mononuclear cell (PBMC) samples from 6 T-ALL cases and 10 normal individuals by using reverse transcriptase-polymerase chain reaction (RT-PCR). The PCR products were further studied by genescan and sequencing analysis. RESULTS: Some TCR Vβ subfamily T cells display mono- or oligoclonal expansions in 3 cases of T-ALL, predominantly in Vβ2, Vβ3, Vβ6, Vβ9, Vβ21 or Vβ24, respectively. Polyclonal expansions of T cells were found in the other three cases, which could also be found in normal samples. CONCLUSION: A part of T cell acute lymphoblastic leukemia cells may arise from a clonal expansion of TCR Vβ subfamily T cell. This method may be useful for the detection of minimal residual disease in clinical study of the disease. 　　［MeSH］　 Receptors, antigen, T-cell; Genes; Leukemia 　　［CLC number］　R733.71; R392.11　　　［Document code］　A 　　［Article FD］　1000-4718(2000)07-0627-06 　　Recently, T cell receptor Vβ gene repertoire and clonality have been studied in patients with leukemia and solid tumors, by assaying the CDR3 size of T cell receptor (TCR) genes with the use of