可吸收医用膜材料预防家兔术后肠粘连效果研究..docxVIP

一种可吸收医用膜材料预防家兔术后肠粘连效果研究钟振东刘洲1 傅荣2※杨玲1廖世平1（1.四川省医学科学院·四川省人民医院实验动物研究所，邮编610072；2四川省人民医院·四川省人民医院整形外科，邮编610072）摘要目的研究一种可吸收医用生物膜材料对家兔术后肠粘连的预防作用。方法 48只家兔按体重随即分为A组（假手术组）和B组（实验组），每组24只，B组家兔复制腹壁缺损/盲肠损伤模型，于损伤腹壁表面缝合可吸收生物膜；A组家兔制作腹腔切割假手术以作对照。分别于术后3天、7天、30天，家兔人道处死后，观察腹壁处肠粘连情况；另取适当大小的粘连处组织，FAA液固定后，石蜡包埋切片，HE染色，于显微镜下观察粘连处病理形态。结果与A组比较， B组家兔肠粘连率明显降低，粘连处炎症、水肿、组织异常增生等病变显著减少。结论该可吸收医用生物材料可明显抑制家兔术后肠粘连。关键词肠粘连可吸收生物膜预防Prevention of an Absorbable Medical Biofilm Material on Rabbit Intestinal Adhesion Abstract Objective To study an absorbable medical biofilm material on rabbit intestinal adhesion prevention. Methods 48 rabbits were randomly divided into group A (sham group) and group B (experimental group) (n = 24), group B rabbits Copy abdominal wall defects / the cecum injury model, the damage to the abdominal wall surface suture absorbable biological film; A group of rabbits produced celiac cutting sham operation as the control. Respectively after 3 days, 7 days, 30 days, rabbits humanely killed after intestinal adhesion observe the abdominal wall at; Another adhesions at the organization of the appropriate size the FAA were fixed, paraffin-embedded sections, HE staining in microscopy observed under pathological adhesions at. Results compared with group A, group B rabbit intestinal adhesion rate was significantly lower, a significant reduction in adhesion at inflammation, edema, tissue dysplasia lesions. Conclusion The absorbable biological materials could significantly inhibit rabbit postoperative intestinal adhesionKey WordsIntestinal Adhesion Absorbable Medical material;Prevention1872年，Bryant 首次报道l 例术后肠粘连引起致命性肠梗阻病人以来，人们为防止术后肠粘连的发生进行了大量动物实验和临床研究。尽管外科无菌术、抗生素的应用和外科技术的改进均有助于预防术后肠粘连的发生，但术后肠粘连仍是腹部手术后并发症中的一大难题，是引起肠梗阻最常见的原因[1]。本实验以成都迪康药业自主研制的一种医用生物材料为研究对象，通过观察其对家兔术后肠粘连的干预效果，初步评价该生物材料预防肠粘连的生物活性，为该生物材料推向临床提供理论依据。1实验材料1.1 实验动物新西兰家兔，SPF级，雌雄各半，体重1.81g-2.25g。由成都达硕生物科技有限公司提供，动物合格证号为SCXK（川）2008-24。1.2受试材料一种可吸收医用生物材料，成都迪康药业提供。1.3实验试剂FAA液：95％乙醇(AR级），批号成都科龙化工试剂厂生产；冰乙酸(AR级），批号成都科龙化工试