荷叶多酚的微波辅助提取工艺优化及其抗氧化活性研究11..doc

荷叶多酚的微波辅助提取工艺优化及其抗氧化活性研究赵晓云 赵博 李志洲 （陕西理工学院化学与环境科学学院 陕西汉中 723001） 1:50.7，乙醇体积分数32.5%，微波作用时间159.4s，荷叶多酚得率可达4.214%。荷叶多酚的抗氧化性研究结果表明0.7mg/mL时对·OH的清除效果优于柠檬酸和Vc；且随着荷叶多酚添加量的增加对油脂的抗氧化效果增强，当添加量大于0.2%时对油脂抗氧化效果优于Vc；加入其他抗氧化剂后荷叶多酚的抗氧化效果明显增强。 关键词：荷叶；多酚；回归正交；抗氧化性 Optimization of the microwave-assisted extract processing of polyphenols in lotus-leaf and oxidation activity ZHAO Xiao-yun, ZHAO Bo, XHING Yuan-yuan, LI Zhi-zhou School of Chemical Environmental Sciences，Shaanxi University of technology Hanzhong Shaanxi 723001 Abstract: The optimum extraction conditions of microwave enhancement extraction of polyphenols from lotus-leaf were optimized by orthogonal regression design and antioxidant activities of the extraction of polyphenols were researched. The results showed that the optimum extraction conditions were the ratio of raw material 1:50.7; ethanol volume fraction 32.5%, the microwave duration 159.4s, the rate of the polyphenols can reach 4.212%. The results of oxidation stability of polyphenols in lotus-leaf showed that the concentration than 0.7 mg/mL of lotus leaf polyphenols samples clearing the ·OH is better than citrate acid and Vc; and as the lotus polyphenols additives to grease antioxidant effects is increasing, grease antioxidant effects of the adding more than 0.2% is better than the Vc,; the antioxidant capacity of polyphenols in lotus-leaf is obviously enhanced after adding other antioxidant. Keywords: lotus-leaf; polyphenols; orthogonal regression; oxidation resistance 植物多酚又名植物单宁，为植物体内的一种次生代谢产物，具有多元酚羟基结构，是多羟基酚类化合物的总称[1]。其主要存在于植物的皮、根、叶、果中，具有多种保健功能，其中最重要的是它的抗氧化功能[2]。它不仅对活性氧自由基具有很强的捕捉能力，而且可以对由氧自由基诱发的生物大分子损伤起到保护作用[3-4]。具有明显的抗突变、抗肿瘤、抗病毒、抗微生物、抗衰老等功能[5-6] 。因而植物多酚在医药、食品、保健品、化妆品等行业中显示出巨大的应用价值。国内外对荷叶黄酮、生物碱类物质的抗氧化活性研究报道较多[7-8]，但对荷叶多酚提取及抗氧化性研究报道较少。本文通过回归正交优化了荷叶多酚的提取工艺，并探索了其对·OH的清除率和对油脂的抗氧化性。 1实验部分 1.1 实验材料与主要仪器 主要原料：荷叶（产地：汉中）没食子酸、三氯化铁、铁氰化钾、盐酸、甲醇、乙醇、丙酮等。 WF-2000微波反应器(上海屹尧分析有限公司)；TU-1800型紫外分光光度计(北京普析通用仪器有限责任公司)；GR-200电子分析天平（日本-AD公司）等。1.2 实验方法1.2.1 没食子酸标准曲线的