介导ctla4ig及iκbα重组腺病毒载体对烧伤创面皮片移植的影响.docVIP

介导CTLA4Ig及IκBα重组腺病毒载体对烧伤创面皮片移植的影响 ［摘要］目的nbsp; 探讨重组CTLA4Ig及IκBα重组腺病毒载体延长烧伤创面皮片移植存活情况。 方法nbsp; 采用Wistar大鼠36只，随机分为3组，1组:正常对照组，2组:用AdCTLA4Ig液室温浸泡转染1h，3组:用Adv CD40LIg-IRES2-CTLA4Ig液室温浸泡转染1h。麻醉后常规背部备皮，在4cm×4cm面积上涂3%凝固汽油2ml，燃烧20s，致大鼠背部皮肤Ⅲ度烧伤，于烧伤后第3天，将所制备1.0cm×2.0cm大小长方形皮瓣移植于创面，加压包扎。观察重组腺病毒载体转染移植物后，在大鼠背部烧伤创面移植人断层皮肤的存活情况的影响。 结果nbsp; 重组腺病毒载体能延长大鼠烧伤创面移植人断层皮肤的存活时间。 结论nbsp; 重组腺病毒可用于延长烧伤创面异基因移植皮肤的存活期。 nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 　　［关键词］ CTLA4Ig;IκBα;腺病毒，重组;异体移植 nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 　　Prolonging the survival of transplanted xenoskin by transfected the CTLA4Ig and IκBαlocally with recombinant adenovirus vector in rats nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 　　Abstract:Objective To observe the effect of recombinant expression vector of CTLA4Ig and IκBαon the survival time of xenoskin graft on burn wound.Methods A total of36Wistar rats established asⅢdegree burn on the back were randomly divided into3groups（n=12in each group）:normal control（the xenoskin without any special treatment），AdCTLA4Ig group（the xenoskin was transfected with AdCTLA4Ig），and Adv CD40LIg-IRES2-CTLA4Ig group ［the xenoskin was transfected with the recombinant adenovirus expression vector of CTLA4Ig and IκBα（Adv CD40LIg-IRES2-CTLA4Ig）that was constructed by means of homorecombination］.After the human skin grafts were transplanted to the burned rats，the survival time and biopsy of grafted skins were observed.Results The recombinant adenovirus expression vector of CTLA4Ig and IκBαcould pro-long the survival time of the grafted xenoskins on burn wounds in rats.Conclusion The recombinant Ad-CTLA4Ig-IRES2 IκBαcould be used to pro-long the survival time of the grafted xenoskins on burn wounds. nbsp;nbsp;nbsp;nbsp;nbsp; 　　Keywords:CTLA4Ig;IκBα;xenoskin;survival nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 　　烧伤治疗的最终目标是及时有效地覆盖封闭创面［1］ 。覆盖封闭创面除自体皮肤外，异基因（异体.异种）移植皮肤是烧伤创面最佳覆盖物，异体皮虽能暂时有效地覆盖封闭创面，但在较短时间内被排斥。CT-LA4Ig是细胞毒性T淋巴细胞相关抗原4（CTLA4）与IgGFc段的基因重组融合蛋白，可阻断抗原特异性T细