冬凌草叶片形态多样性的研究.docVIP

冬凌草叶片形态多样性的研究 【摘要】nbsp; 目的通过对8种不同叶型冬凌草叶片形态特征、叶表皮特征的观察及叶片主要性状的测量，探讨冬凌草种质资源的多样性，为其新品种的选育提供一定的依据。方法对冬凌草叶型多样性进行了观察描述，并采用直尺对叶片主要性状如：叶长、叶宽、叶先端长、宽度进行了测量；对不同叶型冬凌草叶表皮进行了剥离和临时制片，并在光学显微镜下观察了其叶表皮特征。结果不同叶型的冬凌草其表皮细胞的形状、气孔类型均相同，但其叶基形状、叶长、叶宽、叶表皮微形态特征（上表皮细胞和下表皮细胞的数目、气孔长度、宽度及气孔密度等）存在明显的差异。结论冬凌草在长期栽培过程中叶外部形态特征及叶表皮的微形态特征出现了一定的变异。 【关键词】nbsp; 冬凌草 叶型 叶表皮特征 气孔 　　Study on the Diversity of Morphological Characteristics of Leaves of Rabdosia rubescens (Hemls.) Hara 　　Abstract:Objective8 types of leaves of Rabdosia rubescens (Hemls.) were employed to find its germplasm resources diversity by observing the morphological parameters of leaf, the characteristics of leaf epidermis, and measurement its main characters. MethodsThe diversity of leaf type was described; the main characteristics such as leaf length, leaf width, the leaf apex length and width were measured; the characteristics of leaf epidermis were observed by means of light microscope after the leaves were exfoliated and temporary sectioned. ResultsThe form of epidermal cells, the patterns of anticlinal walls, the stomatanbsp; index were anomocytic. But there existed significant difference in the form of leaf base , leaf length, leaf width and micro-morphology characteristics of the leaf epidermis（the cell number of the adaxial epidermis and abaxial epidermis, the stomal length,the stomal width,the stomal density）.ConclusionIt exhibited mutation in morphological characteristics of the leaf and micro-morphology characteristics of the leaf epidermis in a long-term cultivation process. 　　Key words:Rabdosia rubescens (Hemls.) Hara;nbsp; Leaf type;nbsp; Leaf epidermis characteristic;nbsp; Stoma nbsp;nbsp;nbsp; 冬凌草Rabdosia rubescens (Henmsl.) Hara为唇形科香茶菜属多年生植物。因其地上部分含有丰富的冬凌草甲素和乙素，而具有清热解毒,消炎止痛,健胃活血及抗肿瘤之功效。临床实验证明冬凌草对食道癌、贲门癌等疾病有一定的疗效［1］。目前河南省许多制药企业生产的冬凌草含片、冬凌草片、冬凌草糖浆等中成药主要以冬凌草为原料植物。据报道冬凌草药材的年需求量约700万kg。但目前冬凌草的野生资源远远不能满足市场的需求，人工栽培的先进技术及对其新品种的选育势在必行。 nbsp;nbsp;nbsp; 目前国内外对冬凌草的相关研究主要集中在其化学成分、药理作用、临床应用、栽培技术等方面［