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Introduction to Liquid Chromatography Columns System Components Applications Troubleshooting A Brief History of Chromatography 1903: Russian botanist Mikhail Tswett separates plant pigments 1938: Russian scientists Izmailov and Shraiber use “drop chromatography”, later perfected as Thin Layer Chromatography (TLC) by Kirchner in the U.S. 1952: Martin and Synge receive Nobel Prize for “invention of partition chromatography” or plate theory to describe column efficiency 1966: HPLC was first named by Horvath at Yale University but HPLC didn’t “catch on” until the 1970s 1978: W.C. Stills int