e x p r e s s i o n c l o n e sfor e（e x p r e s s i o n c l o n e sfor e）.docVIP

CLONES Basic Plasmid Cloning Restriction enzymes are used to cleave the vector, and cleave a compatible fragments out of the insert. DNA ligase is used to join insert to vector. The mixture is transformed into E. coli, and then the bacteria are cloned. Each clone will have only one of the possible ligation products, because of incompatibility (meaning that if multiple plasmids of the same kind enter a bacterium, all but one of them gets kicked out). Modern cloning vectors have engineered multiple cloning sites. This is the map of pUC19, from the New England BioLabs catalog: Fo