long range p c r sample prep method- europe p m c（long range p c r sample prep method - europe p m c）.docVIP

Experimental methods Preparation of DNA DNA for a bacterial artificial chromosome (bCX98J21) clone was prepared from a single colony by standard alkaline extraction method and purified by equilibrium density gradient fractionation using standard procedures. X chromosome DNA was prepared by flow-sorting X chromosomes from a culture of human lymphoblastoid cell line GM07340. Cells were arrested in metaphase using demecolcine, harvested, treated with hypotonic solution, transferred to polyamine isolation buffer for lysis. Chromosomes were harvested, stained overnight with Hoechst and Chromom