mehlich1 extractionfor macro-and micronurtients（mehlich 1 extractionfor macro - and micronurtients）.docVIP

Mehlich-1 Extraction for Macro- and Micronutrients Rao Mylavarapu and Robert Miller Application and Principle The Mehlich-1 (M1) extraction method was developed by Mehlich in 1953 to primarily determine the relative bioavailability of ortho-phosphate (PO4-P) in soils with acid to neutral pH and with low CEC (10 meq/100g). The M1 extracting reagent comprises of a mixture of 0.05 N HCl and 0.025 N H2SO4 and is also referred to as ‘dilute double acid’ solution. Phosphorus content is determined spectrophotometrically at 882 nm at an acidity of 0.20 M H2SO4 by reacting with ammonium molybdate