花脸蘑多糖LSPb1在喉癌细胞裸鼠移植瘤模型中抑制血管形成.docVIP

花脸蘑多糖LSPb1在喉癌细胞裸鼠移植瘤模型中抑制血管形成 摘要目的：研究花脸蘑多糖（LSPb1）在体内对喉癌血管新生的作用，探究其抗肿瘤的作用机制。方法: ELISA检测体外培养的喉癌Hep-2细胞在低氧环境下分泌血管内皮生长因子（VEGF）的量研究LSPb1移植瘤体积的影响ELISA试剂盒和RT-PCR检测移植瘤内VEGF蛋白和mRNA水平的表达免疫组化定量分析移植瘤组织中新生血管的数量。 结果: 体外实验结果显示，LSPb1能显著抑制VEGF的分泌。体内实验表明，LSPb1能显著抑制Hep-2异种移植裸鼠中瘤体的生长；LSPb1能从抑制VEGF在mRNA水平的表达来抑制其在移植瘤中的含量，进而阻止移植瘤血管的新生；LSPb1能显著降低裸鼠移植瘤组织内的血管密度。 这些研究结果表明，LSPb1作为一个潜在的喉癌治疗药物有望在治疗人喉癌中发挥重要的作用。 关键词: 花脸蘑子实体；多糖；LSPb1；Hep-2；VEGF。Lepista sordida polysaccharides (LSPb1) inhibiting angiogenesis in laryngeal cancer cell xenograft model Abstract LI Mingzhen1, MIAO Susheng1, MAO Xionghui1, ZHAO yu2, SUN ji1 1 Nose Throat Mouth Ward, Department of Head and Neck Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, 150081, China 2 Department of General Surgery, Bayan County People’ Hospital, Bayan, 151800, China 【Abstract】Objective: To study Lepista sordida polysaccharides (LSPb1) for laryngeal cancer angiogenesis in vivo, explore its anti-tumor mechanism. Methods: Use ELISA experimental method to check the vascular endothelial growth factor (VEGF) secreting by laryngeal cancer Hep-2 cells. Xenograft tumor mode to study effect of LSPb1 in vivo laryngeal cancer Hep-2 cells in tumor volume. ELISA kits and RT-PCR experiments were used to detect the expression of VEGF in the tumor protein and mRNA levels. Immunohistochemical methods were used to quantitative analysis of tumor tissue angiogenesis number. Results: In vitro experiments showed that LSPb1 can significantly inhibit the secretion of VEGF. In vivo experiments showed that , LSPb1 could significantly inhibit Hep-2 xenograft tumor growth in nude mice ; LSPb1 able to suppress the expression of VEGF mRNA levels in suppressing tumor in its content , and thus prevent tumor angiogenesis ; LSPb1 can significantly reduce the density of blood vessels within the tumor tissue in nude mice ; These findings suggest that, LSPb1 larynx as a potential therapeutic agent is expected to play an important role in the treatment of huma