Foxq1与Ecadherin蛋白在大肠癌中的表达及临床意义.docVIP

Foxq1与E-cadherin蛋白在大肠癌中的表达及临床意义 骆 赞1，魏正强1*，向 相1，蒙长远1，黎 晖1，汤为学2 （1重庆医科大学附属第一医院胃肠外科 重庆 400016；2重庆市神经病学重点实验室 400016） [摘要] 目的 探讨Foxq1Forkhead box Q1）与E-cadherin在大肠癌中的表达，阐明二者与大肠癌发病及转移的临床病理关系。方法 收集重庆医科大学附属第一医院2012年3月到2012年9月确诊为大肠癌的临床病理标本62例，采用免疫组织化学法检测Foxq1与E-cadherin蛋白在上述标本中的表达水平与定位，应用Western blot检测Foxq1与E-cadherin蛋白的表达水平。结果Foxq1与E-cadherin蛋白 Foxq1在大肠癌组织中高表达，在癌旁组织中次之、正常粘膜组织中最低；E-cadherin蛋白的表达则在癌组织、癌旁组织、正常粘膜组织中梯度升高（P0.05）。二者的表达在大肠癌中呈负相关（P0.05）。结论Foxq1和E-cadherin参与了大肠癌的发生发展，可作为大肠癌早期诊断的联合指标之一，可能为大肠癌的治疗和化疗新药的开发提供理论基础。 [关键词] 大肠癌；Foxq1；E-cadherin蛋白；EMTExpression of Foxq1 and E-cadherin in colorectal cancer and Its Clinical Significance Luo ZAN1，Zhengqiang WEI1，Xiang Xiang1，Hui Li1，Chang Yuan1，Weixue Tang2 Correspondence to: Zhengqiang WEI, E-mail: weizhengq75@ 1Department of General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China 2Chongqing Key Laboratory of Neurology，Chongqing 400016, China Abstract Objective：To study the expression of and E-cadherin in colorectal cancer and clarify clinical pathological relationship between the two and colorectal cancer. Methods：ollecting 62 specimens that have been diagosed as colorectal cancer since March 2012 to September 2012 from Department of General Surgery, The First Affiliated Hospital of Chongqing Medical University . Using immunohistochemistry methods to detect the expression level and location of Foxq1 and E-cadherin. Using Western blot to detect the expression level of Foxq1 and E-cadherin. Results：The positive expression of Foxq1 and E-cadherin were located in cytoplasma. Compared with normal tissues, the expression of foxq1 was significantly increased in colon carcinoma tissues and mild increased in para-carcinoma tissues. However the expression of E-cadherin exhibited a decreased tendency from normal tissues to para-carcinoma tissues and colon carcinoma tissues . Foxq1 and E-cadherin appeared in an obvious negative correlation(P0.05) Conclusion：Foxq1 and E-cadherion do i