高效液相色谱法测定兔血浆中注射用纳米羟基喜树碱的浓度..docVIP

高效液相色谱法测定兔血浆中注射用纳米羟基喜树碱的浓度 陈渝军1, 易以木2(1.武汉市妇女儿童医疗保健中心，湖北武汉 430016；2.华中科技大学同济医学院药学院，湖北武汉 430030) [摘要]目的：建立家兔血浆中注射用纳米羟基喜树碱含量的HPLC测定方法。方法：血浆样品酸化后，提取分析。色谱柱C18 （10 μm， 250 mm×4.6 mm ID），流动相为 10 mmol/L磷酸盐缓冲溶液（pH4.0）-甲醇（50：50），流速1.0 mL/min，柱温30 ℃，检测波长384 nm。以5.0 mg/kg剂量给大白兔耳缘静脉推注纳米羟基喜树碱针，于不同时间点采血测定药物浓度。结果：羟基喜树碱保留时间为8.7 min，定量线形范围为20-8000 ng/mL。血浆中羟基喜树碱的回收率为96.92%-103.51%，日内精密度 ≤ 7.87%，日间精密度 ≤ 11.55%。纳米羟基喜树碱注射后符合三室模型。结论：本法简便实用，定量准确，可满足纳米羟基喜树碱动力学研究的需要。 [关键词]羟基喜树碱；纳米；高效液相色谱；血药浓度 Determination the concentration of injected-nano-hydroxycamptothecin in plasma of rabbits by HPLC CHEN Yu-Jun1, YI Yi-Mu2 (1. Wuhan medical and health center for women and children, Hubei Wuhan, 430016; 2.School of pharmacy, Tongji medical college, Huazhong university of science and technology, Hubei Wuhan, 430030) [Abstract] Objective: To establish a HPLC method for the determination of injected-nano-hydroxycamptothecin in plasma of rabbits. Methods: After acid treatment, plasma samples were injected and measured by abstraction. The Chromatographic column was C18(10 μm， 250 mm×4.6 mm ID). Methannol-10mmol/L phosphate buffer (pH4.0) (50:50) was served as mobile phase at 1.0 mL/min . Detection wavelength was 384 nm. Determination the concentration of injected-nano-hydroxycamptothecin in plasma of rabbits after iv. administration with the dosage of 5.0 mg/kg. Results: The retention time of hydroxycamptothecin was 8.7 min. A good linearity was shown in the concentration range of 20-8000 ng/mL. The recovery was between 96.92% and 103.51%. The intra-day RSD was less than 7.87% and the inter-day RSD was less than 11.55%. The concentration-time curves of injected-nano-hydroxycamptothecin mainline conformed to three-compartment model. Conclusion: This method was simple, practical and accurate. It could be applied in pharmacokinetic study of nano-hydroxycamptothecin. [Key words] hydroxycamptothecin; nano; HPLC; drug concentration in plasma 喜树碱(camptothecin,CPT)是从我国特有的珙垌科植物喜树的树千、树皮和果实中提取的一种具有抗肿瘤作