02序列查询和提交精篇课件.pptVIP

Cluster sizes in UniGene This is a gene with 1 EST associated; the cluster size is 1 Cluster sizes in UniGene This is a gene with 10 ESTs associated; the cluster size is 10 Cluster sizes in UniGene (Human) Cluster size Number of clusters 1 ? 8,100 2 38,200 3-4 23,300 5-8 12,000 9-16 5,600 17-32 3,700 ?500-1000 1,050 ?2000-4000 100 ?8000-16,000 12 ?16,000-30,000 2 UniGene: unique genes via ESTs Conclusion: UniGene is a useful tool to look up information about expressed genes. UniGene displays information about the abundance of a transcript (expressed gene), as well as its regional distribution of expression (e.g. brain vs. liver). Four ways to access DNA and protein sequences [1] Entrez Gene with RefSeq [2] UniGene [3] European Bioinformatics Institute (EBI) and Ensembl (separate from NCBI) [4] ExPASy Sequence Retrieval System (separate from NCBI) Ensembl to access protein and DNA sequences 输入RBP4检索 检索到3条记录 Four ways to access DNA and protein sequences [1] Entrez Gene with RefSeq [2] UniGene [3] European Bioinformatics Institute (EBI) and Ensembl (separate from NCBI) [4] ExPASy Sequence Retrieval System (separate from NCBI) ExPASy to access protein and DNA sequences http://www.expasy.ch/ http://www.expasy.ch/sprot/ 点击SRS检索系统 Example of how to access sequence data: HIV-1 pol There are many possible approaches. Begin at the main page of NCBI, and type an Entrez query: hiv-1 pol Searching for HIV-1 pol: Following the “genome” link yields a manageable three results Example of how to access sequence data: HIV-1 pol For the Entrez query: hiv-1 pol there are about 40,000 nucleotide or protein records (and 100,000 records for a search for “hiv-1”), but these can easily be reduced in two easy steps: --specify the organism, e.g. hiv-1[organism] --limit the output to RefSeq! only 1 RefSeq over 100,000 nucleotide entries for HIV-1 二、序列信息的提交 * * 生物信息学 第二章 序列查询和提交 一、序列信息的查询 Acession numbers 获取序列的四种方法 二、序列信息的提交 一、序列信息的