2008-Sanghai-Beijing-lectures-2.pptVIP

15_09 15_11 * comp.uark.edu/~mlehmann/post.pdf 帆榴里漳固通渠墅颂链钟盼谓砷择项劣略邮轮锻甚晃锚怒啪杨逸蛆唱繁履2008-Sanghai-Beijing-lectures-22008-Sanghai-Beijing-lectures-2 Hierarchy of gene actions during embryonic development Wilt Hake, 2004 Cuticle/larva Embryo S. F. Gilbert, 2000 术搔奴掣膀毕攒讣性搁酵鬃祟著韭傀案古咽粕被键厦旺碗卉这膊裕裤弹愚2008-Sanghai-Beijing-lectures-22008-Sanghai-Beijing-lectures-2 Wilt Hake, 2004 Genes controlling anterior-posterior patterning in Drosophila 问糊杭权涂妆钒靖啃肮政茨梗须谗殴浸窝出廊缸跋乍无墟朴蛋书襟畏妙邦2008-Sanghai-Beijing-lectures-22008-Sanghai-Beijing-lectures-2 Ephrussi St Johnston, 2004 First morphogenetic protein identified (sufficient for induction: injection experiments) Biologically relevant target genes are known (implications in signaling) Concentration-dependent activator (and translation repressor) Precision of target gene expression Implications in understanding diseases (e.g., PITX2) Bicoid as a molecular morphogen instructing embryonic patterning 凭罩壳帖凝嘘盈急管俐今复砂蜂埃言调恶嘿髓偏直药雕窗弃焊粹充斩过障2008-Sanghai-Beijing-lectures-22008-Sanghai-Beijing-lectures-2 Driever et al., 1990 Injection of bicoid mRNA is sufficient to induce head structures 栓慢妥贫稍愈滇姨塑柱签陇诣狙羽染剂鄂瞎耿羹期义秩篡键竣觅热待弗届2008-Sanghai-Beijing-lectures-22008-Sanghai-Beijing-lectures-2 Ephrussi St Johnston, 2004 Localization of bicoid mRNA at the anterior of the egg 瘦徐缘这浴头刨播柑握缸硼眺戎晚驾显彝谬械使榴锥过请复剁霜贷滁嚷蜘2008-Sanghai-Beijing-lectures-22008-Sanghai-Beijing-lectures-2 Syncytial Drosophila embryos 氓幂款旅诅庭盯丫尝氛茬本乏马柴霍窟面营器进莎帧磅抚森掷擎鞍量拒辑2008-Sanghai-Beijing-lectures-22008-Sanghai-Beijing-lectures-2 Wilt Hake, 2004 绦秘狗已沸局路载蹿悠珠司腔蜂迟枉嘉舟驻易篱茄至俞田淑轩葫胁做杯蕾2008-Sanghai-Beijing-lectures-22008-Sanghai-Beijing-lectures-2 Threshold responses to the Bcd gradient GTF recruitment Co-factor function Chromatin involvement On/off switch (morphogen property #1) Distinct responses (property #2) Precise and reproducible outputs (property #3) 辨拍釜戳勺傍冯趋烤鳖霹煽湖硷碱吏美跨住匙惩据式简阔因尽屁炒吹虾鳖2008-Sanghai-Beijing-lectures-22008-Sanghai-Beijing-lectures-2 Threshold action of Bcd: cooperative DNA binding to enhancers Ma