金钱草水提液对小鼠肾脏草酸钙结石形成的干预作用3.28技巧.doc

金钱草水提液对小鼠肾脏草酸钙结石形成的干预作用李静( 胡国全 桂敬强 贺绍君刘德义 陈会良 （安徽科技学院 动物科学学院 安徽省凤阳县东华路9号 233100）摘要：目的 研究金钱草水提液对草酸钙结石小鼠的钙、磷代谢及对肾脏功能及其组织结构的影响，为金钱草在防治草酸钙结石方面的应用提供参考。方法 采用1%乙二醇和1%氯化铵混合溶液自由饮水方式诱发小鼠形成草酸钙结石同时用金钱草水提液作为干预因素，28d试验期结束后，空腹摘除眼球法采血，制备血清，测定血清中钙、磷、尿酸、尿素氮和肌酐的含量，测定肾组织中钙和磷含量，HE染色切片观察肾脏组织结构变化。结果 金钱草干预组小鼠血清钙和肾组织匀浆中钙含量均显著降低（P0.05）；草酸钙结石组血清磷含量极显著升高(P＜0.01）；金钱草干预组肾组织中磷含量显著低于正常对照组 （P0.01）。各组尿酸含量差异不显著；与正常对照组比较，草酸钙结石组尿素氮含量极显著升高（P＜0.01）；与草酸钙结石组相比较，金钱草干预组尿素氮的含量极显著降低（P＜0.01），与空白对照组和草酸钙结石组相比较，金钱草干预组肌酐含量显著降低（P＜0.01）。草酸钙结石组小鼠的肾小管腔中分布大量散在分布的透明草酸钙晶体，肾小球轻度萎缩。金钱草干预组的肾组织中未见明显的草酸钙晶体沉积。结论 金钱草可有效减少草酸钙结石模型小鼠肾脏草酸钙晶体的形成，其作用机制可能与其降钙护肾有关。 关键词：金钱草；草酸钙结石；钙；磷；尿酸；尿素氮；肌酐 The Intervention Effect of Aqueous Extract from Lysimachia Hristinae Hance on Renal Calcium Oxalate Crystallization Formation in Mice LI Jing* , Hu Guo-quan, Gui Jing-qiang, He Shao-jun, Liu De-yi, Chen Hui-liang Jin Er-hui (College of Animal Science, Anhui Science and Technology University, Fengyang, Anhui 233100, China) Abstract: Objective To investigate the intervention effect of aqueous extract from Lysimachia Hristinae Hance on calcium oxalate Stones in mice of calcium and phosphate metabolism, renal function and structure, provide reference for the application Lysimachia Hristinae Hance in preventing calcium oxalate stones. Method The mice with calcium oxalate stone formation were induced by 1% ethylene glycol and 1% ammonium chloride, meanwhile used aqueous extract from Lysimachia Hristinae Hance as intervening factors, .After 28 days medication, the calcium, phosphorus, uric acid, urea nitrogen and creatinine content was measured in serum delete prepared by taking blood through removing the eyes. The calcium content and phosphate content in kidney tissues a was determined. Histological section of kidney were made HE staining. Results That calcium levels in serum and calcium in renal tissue was significantly lower in Lysimachia Christinae Hance intervention group（P0.05）; serum phosphorus levels in Oxalate Calcium Stone group was grea