1110301032姬蓉蓉(1.docVIP

毕业论文 题 目：学 生 姓 名：学 号： 指 导 教 师： 芦光新 专 业 年 级 所在学院和系：完 成 日 期：答 辩 日真菌Marasmius tricolor310b的培养特性及 发酵产漆酶条件的优化 The cultivation of the fungus Marasmius tricolor310b characteristics and fermentation condition of laccase production optimization Rongrong-ji (university of agriculture and animal husbandry in qinghai college of pratacultural science, qinghai xining 810016). Abstract In alpine meadow soil cellulose decomposition fungi strains from Marasmius tricolor310b as the research object, in different temperature, different carbon source, nitrogen source, under different conditions, such as different salt concentration and pH, strain diameter can be determined through cross crossover method, the results show that strain Marasmius tricolor310b at 30 ℃, initial pH 4.8 (partial acid) and sucrose as carbon source and peptone as nitrogen source, grow fastest when salt concentration of 3% or less.Shake bottle of liquid fermentation method, straw powder as substrate respectively under different culture conditions (temperature, concentration of carbon source, nitrogen source, salt, pH), the size of the determination of laccase production live, the results showed that 15 to 25 ℃ with the temperature rising, laccase activity increased, 25 ℃ when the largest producing laccase activity, 25 and 35 ℃ range, with the temperature rising, laccase less activity;Within 20 to 28 ℃ to produce enzyme activity of more than 80%, initial pH value of strain 310 b produced laccase activity has an effect, the smaller the pH is helpful for enzyme production, pH = 4.0 laccase activity, Different carbon source induced strain 310 b produced laccase activity, add sugar can promote strain 310 b secrete laccase, but no significant difference, and add other carbon source can inhibit strains 310 b production of laccase activity, different nitrogen source induced strain 310 b prod