脊髓缺血损伤动物模型的建立.docVIP

脊髓缺血损伤动物模型的建立 作者：管玉龙，刘锋，董培青，万彩红，杨璟 【摘要】 目的本研究拟建立胸降主动脉手术脊髓缺血动物模型，为脊髓保护策略和药物干预研究提供基础。方法实验中采用6只北京农大小型猪。经左侧第五肋间开胸，常温条件下在锁骨下动脉以远阻断降主动脉，30min后阻断开放。动物复苏后，对其下肢行为学临床评分每日进行观察。7日后处死动物取材，进行损伤区域脊髓超微结构观察。结果术中血液动力学平稳。血气结果均维持在正常范围。复苏后1只动物术后每天肌力均为IV 级，7天后处死时肌力IV～V级；其余5只动物术后下肢肌力0 ～I级。电子显微镜超微结构观察显示脊髓较严重的损伤性改变。结论应用小型猪为动物模型，在常温条件下胸降主动脉阻断30min，可以建立急性脊髓缺血术后截瘫模型。 【关键词】 脊髓；缺血；胸主动脉 　　The Establishment of Porcine Model of Spinal Cord Ischemic Injury 　　Abstract: OBJECTIVE This study was designed to establish a porcine model of spinal cord ischemic injury produced by descending thoracic aorta occlusion and provide basis for strategy of spinal protection and pharmaceutical intervention. METHODSSix piglets were used in the experiments supplied by the Beijing College of Agriculture. A standard left thoracotomy was made through the fifth intercostals space. The descending aorta was clamped distal to left subclavian artery at normothermia. Thirty minutes later, the clamps were released. The trachea was extubated once the animals recovered breathing spontaneously. The animals were evaluated daily according to a quantitative behavioral score. On postoperative seventh day the animals were sacrificed and histological analysis for the injury of spinal cord was made. RESULTSHemodynamic data showed all animals were stable before, during and after the procedure. The results of behavioral scoring for each animal demonstrated one animal with paraparesis and other 5 animals with paraplegia. The injury of spinal cord was also demonstrated by ultrastructural measurement.CONCLUSIONA porcine model of spinal cord ischemic injury can be established by descending thoracic aortic clamping thirty minutes at normothermia. 　　Key words：Spinal cord；Ischemia；Thoracic aorta 　　胸主动脉疾病实施外科治疗期间，围术期多种因素可能引起脊髓缺血，术后脊髓并发症一旦发生，直接影响手术效果和患者的生存质量。根据文献报告，多以单纯阻断降主动脉30min为临床界定的安全时限。本研究对实验动物进行胸降主动脉阻断30min后随访观察，建立了脊髓缺血动物模型，可为进一步的脊髓保护策略和药物干预提供基础。 　　1材料与方法 　　1.1实验动物 　　6只北京农大小型猪，体重20 kg～25.9 kg，雌