西洋参二醇皂苷对糖尿病肾病大鼠血常规的影响.docVIP

西洋参二醇皂苷对糖尿病肾病大鼠血常规的影响 【摘要】 目的 探讨西洋参二醇皂苷（PQS）对糖尿病大鼠血常规的影响。方法Wistar大鼠腹腔注射链脲佐菌素（STZ）建立DN模型，按空腹血糖值及体重将大鼠随机分为模型组、西洋参二醇组皂苷小、大剂量组（西洋参二醇组皂苷100，200 mg/kg）、阳性药组（卡托普利10 mg/kg），连续灌胃给药35 d，每10天测定1次血糖，35 d后取全血测白细胞、淋巴细胞、红细胞、血小板、血红蛋白。结果与模型组比较，西洋参二醇皂苷大剂量组大鼠红细胞、血小板、血红蛋白明显降低（Plt;0.01），西洋参二醇皂苷小剂量血红蛋白明显降低（Plt;0.01），红细胞及血小板有降低趋势，无统计学意义；西洋参二醇皂苷大剂量组白细胞明显升高（Plt;0.05）、淋巴细胞有升高的趋势，但无统计学意义，西洋参二醇皂苷小剂量白细胞及淋巴细胞均显著降低（Plt;0.05）。结论 西洋参二醇皂苷能降低糖尿病大鼠红细胞及血小板数、降低血红蛋白含量，不同剂量的西洋参二醇皂苷对白细胞和淋巴细胞影响不同。 【关键词】 西洋参二醇皂苷 糖尿病 链脲佐菌素 常规 　　Effect of Panaxadiol Quinqueloium Saponins on Blood Convention of Diabetic Rats 　　Abstract：ObjectiveTo study the effect of PQS on blood convention of DM rat. MethodsDN model rats were made by STZ, and according to the blood glucose of empty stomach and weight, DN rats were divided into model group、low dose PQS group and large dose PQS group(PQS 100mg/kg,200mg/kg), positive group(Captopril 10mg/kg), detect blood glucose 10 days once time, detect WBC,lymphocyte,RBC,platelet,hemoglobin and blood glucose after 35 days. ResultsCompared with model group, RBC、platelet and hemoglobin of high dose PQS group were depressed（Plt;0.01）, hemoglobin of low dose PQS group were depressed（Plt;0.01）, RBC and platelet have tendency to be depressed, but not statistics significance; WBC of high dose PQS group were stepped up（Plt;0.05）, lymphocyte had tendency to be stepped up、but was not statistics significance; WBC and lymphocyte of low dose PQS group were depressed（Plt;0.05）ConclusionPQS can depress RBC、platelet and hemoglobin of DN rat, different dose PQS have different effect on WBC and lymphocyte of DN rat. 　　Key words：Panaxadiol Quinqueloium Saponins; Diabetes mellitus; Streptozotocin; Blood convention 糖尿病是一种慢性疾病，对全身各个脏器均有损伤，并对血液系统也有影响。西洋参是我国珍贵的中药材，具有抗疲劳、抗缺氧、抗应激等药理作用，中医学认为西洋参有强壮、滋补、活血化瘀功能。西洋参二醇皂苷对糖尿病大鼠血常规的影响，未见报道，本研究组在观察西洋参二醇皂苷对糖尿病肾病大鼠的治疗作用时，发现其对大鼠血常规的有明显影响，现报道如下。 　　1 材料 　　1.1 动物健康雄性Wistar大鼠100只，体重200～220 g，由吉林大学实验动物中心提供，合格证号为（吉）2003－0007。 　　1.2 药品及仪器