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蚯蚓纤溶酶壳聚糖微球的制备及性质 （1河北大学 生命科学院河北 保定 071002 2.河北大学 生物技术研究中心河北 保定 071002） 摘要：关键词：蚯蚓纤溶酶；壳聚糖微球；载药率；包封率；溶出率 中图分类号：文献标码： Preparation of Chitosan Microsphere Loaded Earthworm Fibrinolytic Enzymes and Its Properties WU Jin-xia1,WANG Ying-rui1, TIAN Zhi-peng1, LI Rui1, ZHANG He-ying2 College of Life Science, Hebei University, Baoding 071002,China; 2. Research Center for Biotechnology, Hebei University, Baoding 071002,China) Abstract: To study the preparing methods of chitason microspheres and investigate the influence in drug activity and stability when earthworm fibrinolytic enzymes (EFEs) was as biomacromolecule model drug. The chitosan microspheres were prepared by ultrasonic emulsification method, EFEs was encapsulated and adsorbed into microspheres respectively. The optimized factors were as follows. V(O): V (W) was 15/1，stirring speed was 800 r/m，adding 1mL of Glutaraldehyde，ultrasonic treatment for 5 min. The loaded rate and encapsulation efficiency of the microsphere encapsulated EFEs at these conditions were 52.6% and 48.7% respectively, the microsphere pocessed the sustained releasing property, the total releasing rate in 72 hours were about 65%. Physiological saline was used to dissolve the EFEs from microsphere, the photogram of PAGE indicated that most of the EFEs components could be dissolved, and no obvious changes could be observed. The dissolved EFEs activity was determined by Folin-phenol method, the results showed that the total dissolution rate of the microsphere encapsulated EFEs was 51.5% when dipped in physiological saline for 72 hours. Keywords: earthworm fibrinolytic enzyme; chitosan microsphere; drug loaded rate; encapsulation efficiency; dissolution rate 收稿日期：2011—4—12 基金项目：河北大学博士基金资助项目 第一作者：武金霞1966—），女，，河北大学教授，博士，主要从事生物化学及基因工程药物研究E-mail:wjx6@163.com 血栓病是严重危害人类健康的常见多发病，死亡率高、致残率高。当血栓发生后，最有效的治疗方法就是溶栓，近年来，以蚯蚓纤溶酶（arthworm fibrinolytic enzymes，EFEs）为主要活性成分的溶栓药在临床上发挥着重要作用，如百奥蚓激酶胶囊、普恩复胶囊等。EFEs是一组多组分的丝氨酸蛋白水解酶，其化学本质是蛋白质，不耐酸，多数组分不能耐受胃蛋白酶[1-3]，目前EFE