SDF1对牵张成骨中的表达及其作用机理研究.doc

SDF-1在牵张成骨中的表达及作用机制研究 [摘要]: 目的 方法结果 结论 [关键词]：[Abstract]: Objective Explore the expression and its mechanism of action of stromal cell-derived factor-1 in rat Mandibular bone area in distraction osteogenesis。Methods 40 SD rats were randomly divided into eight groups (n = 5), All the right side of the mandible surface of the skin incision, adjust the shaping and fixed. The left side of the rat mandibular fractures and internal fixation with a titanium plate as controls under. subsequently sacrificed at different points anesthesia time (od, 3 d, 6 d, 9 d, 12 d, 15 d, 18 d, 21 d),, anatomy of rats after bilateral mandible, carefully scrape callus distraction gap on the right side and the left side of the fracture callus For immunohistochemistry chemical tests and ELISA .Results In 40 rats, the normal wound healing, surgery was well tolerated; Immunohistochemical staining showed that, SDF-1 in osteogenic cells and capillaries around the more obvious expression, and the expression in DO freshmen than callus the fracture callus expression is more obvious; ELISA test results showed: SDF-1 in both DO zone or fracture zone, its value was increased three times, and one week after surgery, SDF-1 in the fracture zone the expression of a downward trend, while in the SDF-1 expression in entering the stretch during its rise again, up to five times the original level, slowly declined after the end of the stretch, and the expression of SDF-1, respectively, the two groups 9 d, 12 d, 15d has a statistically significant difference (P 0.05). Conclusions SDF-1 expression level differences in distraction bone area and fracture zones obvious, Laid the foundation for future research SDF-1 expression in distraction and mechanism of bone. [Key words]: Distraction Osteogenesis；Stromal Cell-Derived Factor-1；Enzyme-linked immunosorbent assay; Mechanism 牵张成骨(distraction osteogenesis，DO)是指在截开的骨缝处安置可以施加牵引力的牵张装置来牵张骨段，从而促进骨缝间新生骨组织的形成[1]。近些年随着牵张成骨技术的不断完善，此技术得到了越来越广泛的应用。基质细胞衍生因子-1(Stromal Cell