孕妇静脉血及其新生儿脐血硒水平及谷胱甘肽过氧化物酶活性.docVIP

孕妇静脉血及其新生儿脐血硒水平及谷胱甘肽过氧化物酶活性 与妊娠肝内胆汁淤积症的关系 王竹晨　刘淑芸王竹晨（中山医科大学孙逸仙纪念医院妇产科）；刘淑芸（610041 成都，华西医科大学附属第二医院妇产科） 关键词: 胆汁郁积,肝内；妊娠；硒；谷胱甘肽过氧化酶 　　【摘要】　目的　探讨孕妇静脉血及其新生儿脐血硒水平及谷胱甘肽过氧化物酶（GSH-Px）活性与妊娠肝内胆汁淤积症（ICP）的关系。 方法　采用催化极谱法及5,5＇-二硫代双（二硝基苯甲酸）直接法检测健康未孕妇女30例（健康未孕组）血硒水平及GSH-Px活性；检测ICP孕妇（ICP组）和正常妊娠妇女（正常妊娠组）各30例孕晚期及产后母血、脐血硒水平及母血、脐血GSH-Px活性。 结果　(1)ICP组产前、后母血硒水平分别为（0.038 9±0.009 0）mg/L和（0.046 3±0.009 2）mg/L，GSH-Px活性分别为（59.31±11.42）活力单位(U)和（68.12±11.46） U，正常妊娠组产前、后母血硒水平分别为（0.047 7±0.009 4）mg/L和（0.051 0±0.009 3）mg/L，GSH-Px活性分别为（68.48±10.47） U和（72.67±9.83） U，两组比较，差异均有显著性（P0.005）。（2）ICP组脐血硒水平为（0.038 7±0.009 3）mg/L，GSH-Px活性为（57.66±12.25） U，正常妊娠组脐血硒水平为（0.046 1±0.008 9）mg/L, GSH-Px活性为（67.46±11.93） U，两组比较，均有极显著性差异（P0.005）。（3）血硒水平与GSH-Px活性呈正相关（P0.001）。 结论　ICP患者血硒水平降低，导致GSH-Px活性下降，自由基形成，破坏了肝细胞膜，可能与ICP的发生有关。 Changes of Selenium Concentration and Glutathione Peroxidase Activity in Maternal and Cord Blood in Intrahepatic Cholestasis of Pregnancy WANG Zhuchen（Department of Obstetrics and Gynecology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510120, China）LIU Shuyun. 　　【Abstract】　Objective　To investigate the importance of selenium concentration and glutathione peroxidase(GSH-Px) activity in intrahepatic cholestasis of pregnancy (ICP). Methods　We measured the selenium concentration by a catalytic polorographic method and GSH-Px activity by a 5,5′-dithionbis (2-nitrobenzoic acid) direct method in maternal and cord blood in 30 pregnant women with and without this disease respectively during the last trimester of pregnancy and post partum and in 30 non-pregnant women. Results　Sults (1) The selenium concentration and GSH-Px activity in maternal blood were found to be significantly lower in ICP than in women with normal pregnancies during the last trimester ［(0.038 9±0.009 0) mg/L，(0.047 7±0.009 4) mg/L, P0.001］ ［(59.31±11.42)U,(68.48±10.47)U,P0.002］ and post partum ［(0.046 3±0.009 2)mg/L，(0.051 0±0.009 3)mg/L,P0.05］ ［(68.12±11.46)U,(72.67±9.83)U,P0.05］. (2)The s