不同提取方法对丹桂挥发油成分的影响.docVIP

不同提取方法对丹桂挥发油成分的影响 夏雪娟1 李冠楠2 罗东升1 夏季1 郑炯1 阚建全1* （西南大学食品科学学院1，重庆 400715） （西南大学生物技术学院2，重庆 400715） 摘 要 为了探究不同提取方法对丹桂挥发油成分的影响，同时采用水蒸气蒸馏（SD）、同时蒸馏萃取（SDE）、超临界CO2萃取（SFE-CO2）和顶空固相微萃取（HS-SPME）技术对丹桂挥发油进行提取和GC-MS分析。结果表明：SD和SDE方法提取得到的化合物种类较多，且主要化合物均为酮类和醇类及其氧化物，含量最高的化合物分别为β-紫罗兰酮和植酮。SFE-CO2和HS-SPME得到的化合物种类较少，但主要化合物明显，均为醇类及其氧化物，含量最高的化合物分别为顺-α,α-5-三甲基-5-乙烯基四氢化呋喃-2-甲醇CO2得到的挥发油颜色鲜艳（橙红），香味浓郁。因此，SFE-CO2和HS-SPME更适于丹桂挥发油的提取分析 关键词 丹桂 超临界CO2萃取 顶空固相微萃取 GC-MS 挥发性成分 差异 中图分类号：TQ654 献标码： 文章编号： Analysis of essential oil extracted by different methods from Osmanthus fragrans Xia Xue-juan1 Li Guan-nan2 Luo Dong-sheng1 Xia Ji1 Zheng Jiong 1,3 Kan Jianquan1,3* (College of Food Science Southwest University1, Chongqing 400715, China) (College of Biotechnology Southwest University2, Chongqing 400715, China) Abstract The steam distillation (SD), simultaneous distillation and extraction (SDE), supercritical CO2 extraction (SFE-CO2), and headspace solid-phase micro-extraction (HS-SPME) methods were used to extract the essential oil simultaneously. Furthermore, GC-MS method was used to analysis the chemical composition. More kinds of chemical compositions were obtained by SD and SDE methods. Ketones, alcohols and their oxides were found to be the main chemical components. The major composition were β-Ionone and 6, 10, 14-trimethyl-2-pentadecanone, respectively. Alcohols and their oxides were found to be the main chemical components of essential oil extracted by SFE-CO2 and HS-SPME methods. The major composition were cis-5-ethenyltetrahydro-α, α-5-trimethyl-2-furanmethanol and (E)-furanoid linalool oxide, respectively. The color and smell of essential oil obtained by SD and SDE methods were changed. Comparatively, essential oil extracted by SFE-CO2 method was with fruity-floral aroma and fresh color. Hence, Compared with SD and SDE methods, SFE-CO2 and HS-SPME methods were more suitable for extraction or analysis of O. fragrans ‘Zhushadan’ essential oil. Key words Osma