川芎嗪对过氧化氢诱导大鼠离体培养晶状体氧化损伤的抗氧化作用.doc

川芎嗪对过氧化氢诱导大鼠离体培养晶状体氧化损伤的抗氧化作用 张世华, 李娜，邓新国 Anti-oxidation Effect of Tetramethylpyrazine on Oxidative Damage of Rat Lens Induced by Hydrogen Peroxide in Culture Zhang Shihua, Li Na, Deng Xin-guo State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China Abstract [Objective] To observe the anti-oxidation of tetramethylpyrazine on oxidative damage of rat lens induced by hydrogen peroxide in culture. [Method] 24 SD rats were divided randomly into 8 groups: normal control group, model group treated with hydrogen peroxide and the tetramethylpyrazine groups treated with hydrogen peroxide combined with different concentration tetramethylpyrazines (0.125mg/ml, 0.25mg/ml, 0.5mg/ml, 1mg/ml, 2mg/ml and 4mg/ml).The usage of concentration of hydrogen peroxide (H2O2) was 800μmol/L. The transparent lenses without any injury were taken from SD rat and were cultured under the different conditions for 24 h in vitro. After the lenses were observed by naked eyes, the clear supernatants were gotten from lenses homogenate. The activation levels of superoxide dismutase(SOD) and the content of malon aldehyde(MDA) were measured by photometry method respectively. [Result] The activation levels of SOD test showed that 0.5 mg/mg，1.0 mg/mg and 2.0 mg/mg TMP could increase the activation levels of SOD obviously compared with the model group, P＜0.05 or P＜0.01. The content of MDA test that all concentrations of TMP could obviously decrease the content of MDA compared with model group, P＜0.01.Conclusion] Tetramethylpyrazine might protect lens of rats from oxidative damage, in which could relate with the increasing SOD activation and decreasing the MDA content. Key Words Tetramethylpyrazine(TMP)；ratlens；hydrogen peroxide；Oxidative damage —————— 本课题为广东省中医药管理局资助项目（No.2007095）资助 作者单位：510060广州, 惠州市第一人民医院眼科（张世华， 李娜）；510060广州,中山大学眼科学国家重点实验室，中山大学中山眼科中心（邓新国） 摘要 [目的] 探讨川芎嗪对过氧化氢诱导大鼠离体培养晶状体氧化损伤的抗氧化作用。[方法] SD大鼠24只，随机分8组，即正常对照组、过氧化氢对照组、不同浓度